dc.contributor.author | Milagro Yoldi, Fermín I. | |
dc.contributor.author | Miranda Gómez, Jonatan | |
dc.contributor.author | Portillo Baquedano, María Puy | |
dc.contributor.author | Fernández Quintela, Alfredo | |
dc.contributor.author | Campión Zabalza, Javier | |
dc.contributor.author | Martínez, José Alfredo | |
dc.date.accessioned | 2013-05-16T14:17:15Z | |
dc.date.available | 2013-05-16T14:17:15Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | PLoS ONE 8(1) : (2013) // e54319 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/10108 | |
dc.description.abstract | INTRODUCTION: MicroRNAs (miRNAs) are being increasingly studied in relation to energy metabolism and body composition homeostasis. Indeed, the quantitative analysis of miRNAs expression in different adiposity conditions may contribute to understand the intimate mechanisms participating in body weight control and to find new biomarkers with diagnostic or prognostic value in obesity management. OBJECTIVE: The aim of this study was the search for miRNAs in blood cells whose expression could be used as prognostic biomarkers of weight loss. METHODS: Ten Caucasian obese women were selected among the participants in a weight-loss trial that consisted in following an energy-restricted treatment. Weight loss was considered unsuccessful when <5% of initial body weight (non-responders) and successful when >5% (responders). At baseline, total miRNA isolated from peripheral blood mononuclear cells (PBMC) was sequenced with SOLiD v4. The miRNA sequencing data were validated by RT-PCR. RESULTS: Differential baseline expression of several miRNAs was found between responders and non-responders. Two miRNAs were up-regulated in the non-responder group (mir-935 and mir-4772) and three others were down-regulated (mir-223, mir-224 and mir-376b). Both mir-935 and mir-4772 showed relevant associations with the magnitude of weight loss, although the expression of other transcripts (mir-874, mir-199b, mir-766, mir-589 and mir-148b) also correlated with weight loss. CONCLUSIONS: This research addresses the use of high-throughput sequencing technologies in the search for miRNA expression biomarkers in obesity, by determining the miRNA transcriptome of PBMC. Basal expression of different miRNAs, particularly mir-935 and mir-4772, could be prognostic biomarkers and may forecast the response to a hypocaloric diet. | en |
dc.description.sponsorship | This work has been supported by the Linea Especial LE/97 of the University of Navarra, the RETICS and CIBERobn Schemes funded by Instituto de Salud Carlos III, the Government of the Basque Country (IT-386-10 and IT-572-13) and the University of the Basque Country UPV/EHU (ELDUNANOTEK UFI11/32). | es |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | info:eu-repo/semantics/openAccess | en |
dc.subject | biomarkers | en |
dc.subject | obesity | en |
dc.subject | hypocaloric diet | en |
dc.subject | microRNA | en |
dc.subject | weight loss | en |
dc.title | High-Throughput Sequencing of microRNAs in Peripheral Blood Mononuclear Cells: Identification of Potential Weight Loss Biomarkers | en |
dc.type | info:eu-repo/semantics/article | en |
dc.rights.holder | © 2013 Milagro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0054319 | en |
dc.identifier.doi | 10.1371/journal.pone.0054319 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |
dc.subject.categoria | AGRICULTURAL AND BIOLOGICAL SCIENCES | |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | BIOCHEMISTRY AND MOLECULAR BIOLOGY | |