Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition
dc.contributor.author | Jebari Benslaiman, Shifa | |
dc.contributor.author | Belloso Uribe, Kepa | |
dc.contributor.author | Benito Vicente, Asier | |
dc.contributor.author | Galicia García, Unai | |
dc.contributor.author | Larrea-Sebal, Asier | |
dc.contributor.author | Alloza Moral, Iraide | |
dc.contributor.author | Vandenbroeck, Koen | |
dc.contributor.author | Ostolaza Echabe, Elena Amaya | |
dc.contributor.author | Martín Plágaro, César Augusto | |
dc.date.accessioned | 2020-10-27T09:57:33Z | |
dc.date.available | 2020-10-27T09:57:33Z | |
dc.date.issued | 2020-09-23 | |
dc.identifier.citation | Biomedicines 8(10) : (2020) // Article ID 373 | es_ES |
dc.identifier.issn | 2227-9059 | |
dc.identifier.uri | http://hdl.handle.net/10810/47301 | |
dc.description.abstract | Cardiovascular disease (CVD), the leading cause of mortality worldwide is primarily caused by atherosclerosis, which is promoted by the accumulation of low-density lipoproteins into the intima of large arteries. Multiple nanoparticles mimicking natural HDL (rHDL) have been designed to remove cholesterol excess in CVD therapy. The goal of this investigation was to assess the cholesterol efflux efficiency of rHDLs with different lipid compositions, mimicking different maturation stages of high-density lipoproteins (HDLs) occurring in vivo. Methods: the cholesterol efflux activity of soybean PC (Soy-PC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC:Chol:1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (LysoPC) and DPPC:18:2 cholesteryl ester (CE):LysoPC rHDLs was determined in several cell models to investigate the contribution of lipid composition to the effectiveness of cholesterol removal. Results: DPPC rHDLs are the most efficient particles, inducing cholesterol efflux in all cellular models and in all conditions the effect was potentiated when the ABCA1 transporter was upregulated. Conclusions: DPPC rHDLs, which resemble nascent HDL, are the most effective particles in inducing cholesterol efflux due to the higher physical binding affinity of cholesterol to the saturated long-chain-length phospholipids and the favored cholesterol transfer from a highly positively curved bilayer, to an accepting planar bilayer such as DPPC rHDLs. The physicochemical characteristics of rHDLs should be taken into consideration to design more efficient nanoparticles to promote cholesterol efflux. | es_ES |
dc.description.sponsorship | This work was supported by the Basque Government (Grupos Consolidados IT-1264-19). U.G.-G. was supported by Fundación Biofísica Bizkaia. A.B.-V. was supported by Programa de especialización de Personal Investigador Doctor en la UPV/EHU (2019) 2019–2020. S.J.-B. and A.L.-S. were supported by a grant PIF (2017–2018) and (2019–2020), Gobierno Vasco, respectively. A.L.-S. was partially supported by Fundación Biofísica Bizkaia. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | apoA-I | es_ES |
dc.subject | rHDL | es_ES |
dc.subject | nanodisc | es_ES |
dc.subject | cholesterol efflux | es_ES |
dc.subject | cardiovascular disease | es_ES |
dc.title | Cholesterol Efflux Efficiency of Reconstituted HDL Is Affected by Nanoparticle Lipid Composition | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2020-10-26T14:22:11Z | |
dc.rights.holder | 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2227-9059/8/10/373 | es_ES |
dc.identifier.doi | 10.3390/biomedicines8100373 | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Biokimika eta biologia molekularra |
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Except where otherwise noted, this item's license is described as 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).