Proteinen desnaturalizazio termalaren bidezko analisia konposatu bioeraginkor berriak aurkitzeko

Abstract

Bioactive compounds are either natural or synthetic chemicals that can affect both the cellular and/or physiological activity in living organisms. Included among these are, for example, drugs that can have beneficial effects on health, through the modulation of biological processes. The development of new strategies to search new bioactive compounds and their new biological targets is a high impact challenge. In order to find target proteins and mechanisms of action, several different methodologies have been developed, including the method discussed in this paper. It is based on the analysis of the modifications caused by bioactive compounds on the protein thermal denaturation process. This work describes the basics of this methodology suitable in the search of bioactive compounds as well as the criteria for its implementation.; Konposatu bioeraginkorrak bizidunetan jarduera zelularraren edota fisiologikoaren gain eragin dezaketen substantzia kimiko naturalak zein sintetikoak dira. Horien artean daude, adibidez, farmakoak, prozesu biologikoen modulazioaren bitartez osasunaren gain efektu onuragarriak bideratu ditzaketenak. Konposatu bioeraginkor berrien eta horien itu biologikoak bilatzeko estrategia berrien garapena inpaktu handiko erronka da. Itu proteinak edota ekintza mekanismoak aurkitzeko metodologia ezberdinak garatu dira. Itu proteinak aurkitzeko metodoen artean, lan honetan jorratzen dena daukagu: konposatu bioeraginkorrek proteinen desnaturalizazio termalean eragindako aldaketen azterketan oinarritutako metodoa. Lan honetan, konposatu bioeraginkorrak bilatzeko metodologia horren oinarriak eta haren inplementaziorako irizpideak azaltzen dira.