Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age
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2021-02-09Author
Delgado San Vicente, Diego
Bilbao, Ane Miren
Beitia San Vicente, Maider
Garate, Ane
Sánchez, Pello
González Burguera, Imanol
Isasti Iribar, Amaia
Zuazo Ibarra, Jone
Capetillo González de Zarate, Estibaliz
García del Caño, Gontzal
Sallés Alvira, Joan
Matute Almau, Carlos José
Sánchez, Mikel
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International Journal of Molecular Sciences 22(4) : (2021) // Article ID 1725
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Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age
by Diego Delgado
1, Ane Miren Bilbao
2, Maider Beitia
1, Ane Garate
1, Pello Sánchez
1, Imanol González-Burguera
3,4, Amaia Isasti
4,5, Maider López De Jesús
4,5,6, Jone Zuazo-Ibarra
7, Alejandro Montilla
7 [OrcID] , María Domercq
7 [OrcID] , Estibaliz Capetillo-Zarate
7,8, Gontzal García del Caño
3,4 [OrcID] , Joan Sallés
4,5,6, Carlos Matute
7 and Mikel Sánchez
1,2,*
1
Advanced Biological Therapy Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain
2
Arthroscopic Surgery Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain
3
Department of Neurosciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01008 Vitoria-Gasteiz, Spain
4
Bioaraba, Neurofarmacología Celular y Molecular, 01008 Vitoria-Gasteiz, Spain
5
Department of Pharmacology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01008 Vitoria-Gasteiz, Spain
6
Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), 28029 Madrid, Spain
7
Achucarro Basque Center for Neuroscience, CIBERNED and Departamento de Neurociencias, Universidad del País Vasco (UPV/EHU), 48940 Leioa, Spain
8
IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Francesca Santilli
Int. J. Mol. Sci. 2021, 22(4), 1725; https://doi.org/10.3390/ijms22041725
Received: 24 November 2020 / Revised: 12 January 2021 / Accepted: 3 February 2021 / Published: 9 February 2021
(This article belongs to the Section Molecular Endocrinology and Metabolism)
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Abstract
Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor’s health status and age. This work aims to prove the effect of PRP on cellular models of the CNS, considering the differences between PRP from young and elderly donors. Two different PRP pools were prepared from donors 65–85 and 20–25 years old. The cellular and molecular composition of both PRPs were analyzed. Subsequently, the cellular response was evaluated in CNS in vitro models, studying proliferation, neurogenesis, synaptogenesis, and inflammation. While no differences in the cellular composition of PRPs were found, the molecular composition of the Young PRP showed lower levels of inflammatory molecules such as CCL-11, as well as the presence of other factors not found in Aged PRP (GDF-11). Although both PRPs had effects in terms of reducing neural progenitor cell apoptosis, stabilizing neuronal synapses, and decreasing inflammation in the microglia, the effect of the Young PRP was more pronounced. In conclusion, the molecular composition of the PRP, conditioned by the age of the donors, affects the magnitude of the biological response.