Abstract
The first cartographic representations of the Paraná delta have been used to characterize the elements that comprised the archaeological site of Fort Sancti Spiritus, an iconic place in the beginnings of the colonization process initiated by the crown of Castile in the southern sector of the Plata drainage basin. The maps of Sebastian Cabot, Sancho Gutiérrez, and Ruy Díaz de Guzmán are compared with written documentation, other maps from the same time, and the information obtained in the latest archaeological studies. This comparative analysis demonstrates the potential of historical cartography as a primary source to enlarge on or nuance knowledge of the initial stages of the conquest and colonization of this region.