BackExaminar por departamento (eus.) "Biokimika eta biologia molekularra"
Now showing items 1-20 of 59
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A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold
(Public Library Science, 2013-08)Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ... -
Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells
(Nature Publishing Group, 2015-09-08)Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ... -
Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity
(Public Library of Science, 2011-02-23)Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ... -
Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment
(Public Library Science, 2014-11-11)Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ... -
Analysis of SUMOylated proteins using SUMO-traps
(Nature Publishing Group, 2013-04-22)SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ... -
Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity
(Public Library Science, 2014-10-20)Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although ... -
ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP
(Public Library of Science, 2013)In this study we have investigated the role of extracellular ATP on thrombin induced-platelet aggregation (TIPA) in washed human platelets. ATP inhibited TIPA in a dose-dependent manner and this inhibition was abolished ... -
Ca2+ Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin (ACT) Endocytosis. Cell Physiology and Expression of the CD11b/CD18 Integrin Major Determinants of the Entry Route
(Public Library Publishing, 2013-09)Humans infected with Bordetella pertussis, the whooping cough bacterium, show evidences of impaired host defenses. This pathogenic bacterium produces a unique adenylate cyclase toxin (ACT) which enters human phagocytes and ... -
Calpain-Mediated Processing of Adenylate Cyclase Toxin Generates a Cytosolic Soluble Catalytically Active N-Terminal Domain
(Public Library Publishing, 2013-06)Bordetella pertussis, the whooping cough pathogen, secretes several virulence factors among which adenylate cyclase toxin (ACT) is essential for establishment of the disease in the respiratory tract. ACT weakens host ... -
Caracterización estructural de complejos ribosomales de iniciación y de pre-translocación mediante microscopía electrónica
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2012-07-04)Los ribosomas son complejos macromoleculares altamente organizados responsables de la síntesis de proteínas en todos los organismos en un proceso llamado traducción. El término traducción hace referencia a que el lenguaje ... -
Characterisation of patients with brachydactyly type E associated or not with hormonal resistance: clinical, genetic and radiological approach
(2015-11-16)La braquidatilia de tipo E es una infrecuente entidad clínica de compleja caracterización, que puede aparecer aislada o en algunas formas sindrómicas. Su infrecuencia conlleva la posibilidad de diagnosticar de forma errónea ... -
Conformational Sampling and Nucleotide-Dependent Transitions of the GroEL Subunit Probed by Unbiased Molecular Dynamics Simulations
(Public Library of Science, 2011-03-10)GroEL is an ATP dependent molecular chaperone that promotes the folding of a large number of substrate proteins in E. coli. Large-scale conformational transitions occurring during the reaction cycle have been characterized ... -
Control of gene expression by modulated self-assembly
(Oxford University Press, 2011-09)Numerous transcription factors self-assemble into different order oligomeric species in a way that is actively regulated by the cell. Until now, no general functional role has been identified for this widespread process. ... -
Degradación de alcanos mediante Alcanivorax venutensis inmovilizada en hidrogeles adhesivos y biodegradables
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2011-01-27)El petróleo y sus derivados son los mayores contaminantes del medio marino. Además puntualmente se producen grandes vertidos accidentales como el que en Noviembre de 2002 produjo un desastre ecológico sin precedentes en ... -
Delivery of small nucleic acids by conjugation to carbohydrates and lipids as novel research and therapeutic tools
(2014-03-12)Small nucleic acids present a great potential to silence specific genes or inhibit the biological activity of specific proteins. Unfortunately, their poor cellular accessibility presents a big challenge for developing an ... -
Desarrollo de un nuevo soporte polímerico con componentes de matriz extracelular neural para su aplicación en la diferenciación "in vitro" de distintas células primarias
(2012-07-11)Este trabajo de Tesis Doctoral presenta el desarrollo, la caracterización y la validación de un nuevo soporte polimérico capaz de inducir la diferenciación neuronal in vitro, combinando técnicas de fotolitografía y de ... -
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Development of soft and rigid membrane nanotemplates for the mechanistic analysis of Dynamin I action on curved surfaces
(2015-03-26)En los últimos años se ha ido desvelando el rol activo de la curvaturade membrana en los procesos de reorganización de membranas celulares. Lamayoría de las membranas celulares presentan una cierta curvatura; dichacurvatura ... -
DNA methylation epigenotypes in breast cancer molecular subtypes
(BioMed Central, 2010-09-29)Introduction: Identification of gene expression-based breast cancer subtypes is considered a critical means of prognostication. Genetic mutations along with epigenetic alterations contribute to gene-expression changes ... -
Estudio de la regulación de PHD1 Y PHD3 por SUMO
(2016-07-06)Las PHDs (Prolyl-Hydroxylase Domain containing proteins) se identificaron inicialmente como sensores de oxígeno, capaces de regular la estabilidad de del factor de transcripción HIF (Hypoxia Inducible Factor), implicado ...