BackExaminar por departamento (eus.) "Biokimika eta biologia molekularra"
Now showing items 1-20 of 165
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A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold
(Public Library Science, 2013-08)Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ... -
A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
(Nature Publishing, 2018-09-17)Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects ... -
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
(Nature Publishing Group, 2019-12)N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ... -
Activation of the DnaK-ClpB Complex is Regulated by the Properties of the Bound Substrate
(Nature Publishing, 2018-04-11)The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which ... -
Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells
(Nature Publishing Group, 2015-09-08)Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ... -
Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity
(Public Library of Science, 2011-02-23)Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ... -
Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment
(Public Library Science, 2014-11-11)Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ... -
An E2F7-Dependent Transcriptional Program Modulates DNA Damage Repair And Genomic Stability
(Oxford University Press, 2018-05-18)The cellular response to DNA damage is essential for maintaining the integrity of the genome. Recent evidence has identified E2F7 as a key player in DNA damage-dependent transcriptional regulation of cell-cycle genes. ... -
Analysis of SUMOylated proteins using SUMO-traps
(Nature Publishing Group, 2013-04-22)SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ... -
Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity
(Public Library Science, 2014-10-20)Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although ... -
Application of Solid Lipid Nanoparticles to Improve the Efficiency of Anticancer Drugs
(MDPI, 2019-03-22)Drug delivery systems have opened new avenues to improve the therapeutic effects of already-efficient molecules. Particularly, Solid Lipid Nanoparticles (SLNs) have emerged as promising nanocarriers in cancer therapy. SLNs ... -
Atomistic Insights of Calmodulin Gating of Complete Ion Channels
(MDPI, 2020-02-14)Intracellular calcium is essential for many physiological processes, from neuronal signaling and exocytosis to muscle contraction and bone formation. Ca<sup>2+</sup> signaling from the extracellular medium depends both on ... -
ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP
(Public Library of Science, 2013)In this study we have investigated the role of extracellular ATP on thrombin induced-platelet aggregation (TIPA) in washed human platelets. ATP inhibited TIPA in a dose-dependent manner and this inhibition was abolished ... -
Bases estructurales del transporte de Mg2+ mediado por los factores homeostáticos CNNM2 y CNNM4, y de su interacción oncogénica con las fosfatasas PRL.
El Magnesio, segundo catión intracelular más abundante, es un ión imprescindible en diversas funciones vitales como la neurotransmisión, la conductancia cardiaca o la síntesis de ATP. Este papel biológico trascendental ... -
BFL1 Modulates Apoptosis at the Membrane Level through a Bifunctional And Multimodal Mechanism Showing Key Differences With BCLXL
(Springer Nature, 2018-12-18)BFL1 is a relatively understudied member of the BCL2 protein family which has been implicated in the pathogenesis andchemoresistance of a variety of human cancers, including hematological malignancies and solid tumours. ... -
Bordetella pertussis-en adenilato ziklasa toxina: makrofagoetan eragindako mintz-iragazkortasun eta heriotz-zelularraren ikerketa
(2017-05-24)La toxina adenilato ciclasa(ACT) es uno de los factores de virulencia mas importantes de Bordetella pertussis, la bacteria causante de la tos ferina. ACT tiene dos actividades perjudiciales para la celul la actividad ... -
Ca2+ Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin (ACT) Endocytosis. Cell Physiology and Expression of the CD11b/CD18 Integrin Major Determinants of the Entry Route
(Public Library Publishing, 2013-09)Humans infected with Bordetella pertussis, the whooping cough bacterium, show evidences of impaired host defenses. This pathogenic bacterium produces a unique adenylate cyclase toxin (ACT) which enters human phagocytes and ... -
Calpain-Mediated Processing of Adenylate Cyclase Toxin Generates a Cytosolic Soluble Catalytically Active N-Terminal Domain
(Public Library Publishing, 2013-06)Bordetella pertussis, the whooping cough pathogen, secretes several virulence factors among which adenylate cyclase toxin (ACT) is essential for establishment of the disease in the respiratory tract. ACT weakens host ... -
Caracterización estructural de complejos ribosomales de iniciación y de pre-translocación mediante microscopía electrónica
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2012-07-04)Los ribosomas son complejos macromoleculares altamente organizados responsables de la síntesis de proteínas en todos los organismos en un proceso llamado traducción. El término traducción hace referencia a que el lenguaje ... -
Ceramide and ceramide 1-phosphate in health and disease
(BioMed Central, 2010-02)Sphingolipids are essential components of cell membranes, and many of them regulate vital cell functions. In particular, ceramide plays crucial roles in cell signaling processes. Two major actions of ceramides are the ...