Now showing items 1-20 of 297

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      A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold 

      Rendón Ramírez, Adela; Shukla, Manish; Oda, Masataka; Chakraborty, Sandeep; Minda, Renu; Dandekar, Abhaya M.; Asgeirsson, Bjarni; Goñi Urcelay, Félix María; Rao, Basuthkar J. (Public Library Science, 2013-08)
      Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ...
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      A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis 

      Barth, Nicole D.; Subirós Funosas, Ramón; Mendive Tapia, Lorena; Duffin, Rodger; Shields, Mario A.; Cartwright, Jennifer A.; Troeira Henriques, Sónia; Sot, Jesús; Goñi Urcelay, Félix María; Lavilla, Rodolfo; Marwick, John A.; Vermeren, Sonja; Rossi, Adriano G.; Egeblad, Mikala; Dransfield, Ian; Vendrell, Marc (Nature, 2020-08-12)
      Programmed cell death or apoptosis is a central biological process that is dysregulated in many diseases, including inflammatory conditions and cancer. The detection and quantification of apoptotic cells in vivo is hampered ...
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      A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines 

      Peris, Leticia; Bisbal, Mariano; Martínez Hernández, José; Saoudi, Yasmina; Jonckheere, Julie; Rolland, Marta; Sebastien, Muriel; Brocard, Jacques; Denarier, Eric; Bosc, Christophe; Guerin, Christophe; Gory-Fauré, Sylvie; Deloulme, Jean Christophe; Lanté, Fabien; Arnal, Isabelle; Buisson, Alain; Goldberg, Yves; Blanchoin, Laurent (Nature Publishing, 2018-09-17)
      Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects ...
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      A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation 

      Castellanos Rubio, Ainara; Santín Gómez, Izortze; Olazagoitia Garmendia, Ane; Romero Garmendia, Irati; Jauregi Miguel, Amaia; Legarda, Maria; Bilbao Catalá, José Ramón (Nature Publishing Group, 2019-12)
      N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ...
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      A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes 

      Ramírez Sánchez, Juan Manuel; Prieto Agujeta, Gorka; Olazabal Herrero, Ane; Borràs, Eva; Fernandez-Vigo, Elvira; Alduntzin Egizurain, Unai; Osinalde Moraleja, Nerea; Beaskoetxea Lejarzegi, Javier; Lectez, Benoit; Aloria Escolastico, Kerman; Rodríguez Pérez, José Antonio; Paradela, Alberto; Sabidó, Eduard; Muñoz, Javier; Corrales, Fernando; Arizmendi Bastarrika, Jesús María; Mayor Martínez, Ugo (MDPI, 2021-05-03)
      The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly ...
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      A Systematic Approach to Assess the Activity and Classification of PCSK9 Variants 

      Belloso Uribe, Kepa; Chemello, Kevin; Larrea Sebal, Asier; Benito Vicente, Asier; Bourane, Steeve; Jaafar, Ali K.; Lambert, Gilles; Martín Plágaro, César Augusto (MDPI, 2021-12-18)
      Background: Gain of function (GOF) mutations of PCSK9 cause autosomal dominant familial hypercholesterolemia as they reduce the abundance of LDL receptor (LDLR) more efficiently than wild-type PCSK9. In contrast, PCSK9 ...
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      Aberrant Synaptic PTEN in Symptomatic Alzheimer's Patients May Link Synaptic Depression to Network Failure 

      Díaz González, Marta; Buberman, Assaf; Morales Fuciños, Miguel; Ferrer, Isidro; Knafo Farhi, Dina Shira (Frontiers Media, 2021-05-11)
      In Alzheimer's disease (AD), Amyloid beta (A beta) impairs synaptic function by inhibiting long-term potentiation (LTP), and by facilitating long-term depression (LTD). There is now evidence from AD models that A beta ...
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      Activation of the DnaK-ClpB Complex is Regulated by the Properties of the Bound Substrate 

      Fernández Higuero, José Ángel; Aguado Martínez, Alejandra; Perales Calvo, Judit; Moro Pérez, Fernando; Muga Villate, Arturo (Nature Publishing, 2018-04-11)
      The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which ...
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      Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells 

      Martín Plágaro, César Augusto; Etxaniz Iriondo, Asier; Belloso Uribe, Kepa; Etxebarria Gallego, Aitor; González Bullón, David; Arlucea de Jaureguizar, Jon Andoni; Goñi Urcelay, Félix María; Arechaga Martínez, Juan Miguel; Ostolaza Echabe, Elena Amaya (Nature Publishing Group, 2015-09-08)
      Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ...
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      Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity 

      Martín Plágaro, César Augusto; Belloso Uribe, Kepa; Gómez Bilbao, Geraxane; Ostolaza Echabe, Elena Amaya (Public Library of Science, 2011-02-23)
      Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ...
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      Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment 

      Echevarria Gallego, Aitor; Benito Vicente, Asier; Ostolaza Echabe, Elena Amaya; Martín Plágaro, César Augusto (Public Library Science, 2014-11-11)
      Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ...
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      Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments 

      Rujas Díez, Edurne; Insausti González, Sara; Leaman, Daniel P.; Carravilla Palomanes, Pablo; González Resines, Saul; Monceaux, Valérie; Sánchez Eugenia, Rubén; García Porras, Miguel; Iloro, Ibon; Zhang, Lei; Elortza, Felix; Julien, Jean Philippe; Sáez Cirión, Asier; Zwick, Michael B.; Eggeling, Christian; Ojida, Akio; Domene, Carmen; Caaveiro, Jose M.M.; Nieva Escandón, José Luis (Cell Press, 2020-08-18)
      The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ...
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      Agricultural Soils Amended with Thermally-Dried Anaerobically-Digested Sewage Sludge Showed Increased Risk of Antibiotic Resistance Dissemination 

      Jauregi, Leire; Epelde Sierra, Lur; Alcorta Calvo, Miren Itziar; Garbisu Crespo, Carlos (Frontiers Media, 2021-04-28)
      The application of sewage sludge (SS) to agricultural soil can help meet crop nutrient requirements and enhance soil properties, while reusing an organic by-product. However, SS can be a source of antibiotic resistance ...
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      An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles 

      Madsen, Thomas D.; Hansen, Lasse H.; Hintze, John; Ye, Zilu; Jebari Benslaiman, Shifa; Andersen, Daniel B.; Joshi, Hiren J.; Ju, Tongzhong; Goetze, Jens P.; Martin, Cesar; Rosenkilde, Mette M.; Holst, Jens J.; Kuhre, Rune E.; Goth, Christoffer K.; Vakhrushev, Sergey Y.; Schjoldager, Katrine T. (Nature, 2020-08-20)
      Peptide hormones and neuropeptides encompass a large class of bioactive peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure. Here, we execute a focused ...
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      An E2F7-Dependent Transcriptional Program Modulates DNA Damage Repair And Genomic Stability 

      Mitxelena Sánchez, Jone; Apraiz García, Aintzane; Vallejo Rodríguez, Jon; García Santisteban, Iraia; Fullaondo Elordui-Zapaterieche, Asier; Alvarez Fernández, Mónica; Marcos, Marcos; Zubiaga Elordieta, Ana María (Oxford University Press, 2018-05-18)
      The cellular response to DNA damage is essential for maintaining the integrity of the genome. Recent evidence has identified E2F7 as a key player in DNA damage-dependent transcriptional regulation of cell-cycle genes. ...
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      An Epilepsy-Causing Mutation Leads to Co-Translational Misfolding of the Kv7.2 Channel 

      Urrutia Iñiguez, Janire; Aguado Martínez, Alejandra; Gomis Pérez, Carolina; Muguruza Montero, Arantza; Rodríguez Ballesteros, Oscar; Zhang, Jiaren; Nuñez Viadero, Eider; Malo de la Fuente, Covadonga; Chung, Hee Jung; Leonardo Liceranzu, Aritz; Bergara Jauregui, Aitor; Villarroel Muñoz, Álvaro (Springer, 2021-05-21)
      BACKGROUND: The amino acid sequence of proteins generally carries all the necessary information for acquisition of native conformations, but the vectorial nature of translation can additionally determine the folding outcome. ...
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      Analysis of SUMOylated proteins using SUMO-traps 

      Da Silva Ferrada, Elisa; Xolalpa, Wendy; Lang, Valerie; Aillet, Fabienne; Martín-Ruiz, Itziar; De la Cruz-Herrera, Carlos F.; Lopitz Otsoa, Fernando; Carracedo Pérez, Arkaitz; Goldenberg, Seth J.; Rivas, Carmen; England, Patrick; Rodríguez, Manuel S. (Nature Publishing Group, 2013-04-22)
      SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ...
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      Analysis on the Characterization of Multiphoton Microscopy Images for Malignant Neoplastic Colon Lesion Detection under Deep Learning Methods 

      Terradillos Fernández, Elena; López Saratxaga, Cristina; Mattana, Sara; Cicchi, Riccardo; Pavone, Francesco S.; Andraka Rueda, Nagore; Glover, Benjamin J.; Arbide del Río, Nagore; Velasco Arteche, Jacques; Etxezarraga Zuluaga, María Carmen; Picón Ruiz, Artzai (Wolters Kluwer, 2021-06-21)
      Background: Colorectal cancer has a high incidence rate worldwide, with over 1.8 million new cases and 880,792 deaths in 2018. Fortunately, its early detection significantly increases the survival rate, reaching a cure ...
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      Antibiotic Resistance in Agricultural Soil and Crops Associated to the Application of Cow Manure-Derived Amendments from Conventional and Organic Livestock Farms 

      Jauregi, Leire; Epelde Sierra, Lur; Alcorta Calvo, Miren Itziar; Garbisu Crespo, Carlos (Frontiers Media, 2021-02-23)
      he application of organic amendments to agricultural soil can enhance crop yield, while improving the physicochemical and biological properties of the recipient soils. However, the use of manure-derived amendments as ...
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      Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity 

      Orzáez, Mar; Sancho, Mónica; Marchán, Sandra; Mondragón, Laura; Montava, Rebeca; García Valero, Juan; Landeta Díaz, Olatz; Basáñez Asúa, Gorka; Carbajo, Rodrigo J.; Pineda-Lucena, Antonio; Bujons, Jordi; Moure, Alejandra; Messeguer, Angel; Lagunas, Carmen; Herrero, Carmen; Pérez- Payá, Enrique (Public Library Science, 2014-10-20)
      Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although ...