Now showing items 1-20 of 197

    • Thumbnail

      A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold 

      Rendón Ramírez, Adela; Shukla, Manish; Oda, Masataka; Chakraborty, Sandeep; Minda, Renu; Dandekar, Abhaya M.; Asgeirsson, Bjarni; Goñi Urcelay, Félix María; Rao, Basuthkar J. (Public Library Science, 2013-08)
      Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ...
    • Thumbnail

      A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis 

      Barth, Nicole D.; Subirós Funosas, Ramón; Mendive Tapia, Lorena; Duffin, Rodger; Shields, Mario A.; Cartwright, Jennifer A.; Troeira Henriques, Sónia; Sot, Jesús; Goñi Urcelay, Félix María; Lavilla, Rodolfo; Marwick, John A.; Vermeren, Sonja; Rossi, Adriano G.; Egeblad, Mikala; Dransfield, Ian; Vendrell, Marc (Nature, 2020-08-12)
      Programmed cell death or apoptosis is a central biological process that is dysregulated in many diseases, including inflammatory conditions and cancer. The detection and quantification of apoptotic cells in vivo is hampered ...
    • Thumbnail

      A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines 

      Peris, Leticia; Bisbal, Mariano; Martínez Hernández, José; Saoudi, Yasmina; Jonckheere, Julie; Rolland, Marta; Sebastien, Muriel; Brocard, Jacques; Denarier, Eric; Bosc, Christophe; Guerin, Christophe; Gory-Fauré, Sylvie; Deloulme, Jean Christophe; Lanté, Fabien; Arnal, Isabelle; Buisson, Alain; Goldberg, Yves; Blanchoin, Laurent (Nature Publishing, 2018-09-17)
      Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects ...
    • Thumbnail

      A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation 

      Castellanos Rubio, Ainara; Santín Gómez, Izortze; Olazagoitia Garmendia, Ane; Romero Garmendia, Irati; Jauregi Miguel, Amaia; Legarda, Maria; Bilbao Catalá, José Ramón (Nature Publishing Group, 2019-12)
      N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches ...
    • Thumbnail

      Activation of the DnaK-ClpB Complex is Regulated by the Properties of the Bound Substrate 

      Fernández Higuero, José Ángel; Aguado Martínez, Alejandra; Perales Calvo, Judit; Moro Pérez, Fernando; Muga Villate, Arturo (Nature Publishing, 2018-04-11)
      The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which ...
    • Thumbnail

      Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells 

      Martín Plágaro, César Augusto; Etxaniz Iriondo, Asier; Belloso Uribe, Kepa; Etxebarria Gallego, Aitor; González Bullón, David; Arlucea de Jaureguizar, Jon Andoni; Goñi Urcelay, Félix María; Arechaga Martínez, Juan Miguel; Ostolaza Echabe, Elena Amaya (Nature Publishing Group, 2015-09-08)
      Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ...
    • Thumbnail

      Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity 

      Martín Plágaro, César Augusto; Belloso Uribe, Kepa; Gómez Bilbao, Geraxane; Ostolaza Echabe, Elena Amaya (Public Library of Science, 2011-02-23)
      Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ...
    • Thumbnail

      Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment 

      Echevarria Gallego, Aitor; Benito Vicente, Asier; Ostolaza Echabe, Elena Amaya; Martín Plágaro, César Augusto (Public Library Science, 2014-11-11)
      Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ...
    • Thumbnail

      Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments 

      Rujas Díez, Edurne; Insausti González, Sara; Leaman, Daniel P.; Carravilla Palomanes, Pablo; González Resines, Saul; Monceaux, Valérie; Sánchez Eugenia, Rubén; García Porras, Miguel; Iloro, Ibon; Zhang, Lei; Elortza, Felix; Julien, Jean Philippe; Sáez Cirión, Asier; Zwick, Michael B.; Eggeling, Christian; Ojida, Akio; Domene, Carmen; Caaveiro, Jose M.M.; Nieva Escandón, José Luis (Cell Press, 2020-08-18)
      The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ...
    • Thumbnail

      An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles 

      Madsen, Thomas D.; Hansen, Lasse H.; Hintze, John; Ye, Zilu; Jebari Benslaiman, Shifa; Andersen, Daniel B.; Joshi, Hiren J.; Ju, Tongzhong; Goetze, Jens P.; Martin, Cesar; Rosenkilde, Mette M.; Holst, Jens J.; Kuhre, Rune E.; Goth, Christoffer K.; Vakhrushev, Sergey Y.; Schjoldager, Katrine T. (Nature, 2020-08-20)
      Peptide hormones and neuropeptides encompass a large class of bioactive peptides that regulate physiological processes like anxiety, blood glucose, appetite, inflammation and blood pressure. Here, we execute a focused ...
    • Thumbnail

      An E2F7-Dependent Transcriptional Program Modulates DNA Damage Repair And Genomic Stability 

      Mitxelena Sánchez, Jone; Apraiz García, Aintzane; Vallejo Rodríguez, Jon; García Santisteban, Iraia; Fullaondo Elordui-Zapaterieche, Asier; Alvarez Fernández, Mónica; Marcos, Marcos; Zubiaga Elordieta, Ana María (Oxford University Press, 2018-05-18)
      The cellular response to DNA damage is essential for maintaining the integrity of the genome. Recent evidence has identified E2F7 as a key player in DNA damage-dependent transcriptional regulation of cell-cycle genes. ...
    • Thumbnail

      Analysis of SUMOylated proteins using SUMO-traps 

      Da Silva Ferrada, Elisa; Xolalpa, Wendy; Lang, Valerie; Aillet, Fabienne; Martín-Ruiz, Itziar; De la Cruz-Herrera, Carlos F.; Lopitz Otsoa, Fernando; Carracedo Pérez, Arkaitz; Goldenberg, Seth J.; Rivas, Carmen; England, Patrick; Rodríguez, Manuel S. (Nature Publishing Group, 2013-04-22)
      SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ...
    • Thumbnail

      Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity 

      Orzáez, Mar; Sancho, Mónica; Marchán, Sandra; Mondragón, Laura; Montava, Rebeca; García Valero, Juan; Landeta Díaz, Olatz; Basáñez Asúa, Gorka; Carbajo, Rodrigo J.; Pineda-Lucena, Antonio; Bujons, Jordi; Moure, Alejandra; Messeguer, Angel; Lagunas, Carmen; Herrero, Carmen; Pérez- Payá, Enrique (Public Library Science, 2014-10-20)
      Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although ...
    • Thumbnail

      Application of Solid Lipid Nanoparticles to Improve the Efficiency of Anticancer Drugs 

      Bayón Cordero, Laura; Alcorta Calvo, Miren Itziar; Arana Urbieta, Lide (MDPI, 2019-03-22)
      Drug delivery systems have opened new avenues to improve the therapeutic effects of already-efficient molecules. Particularly, Solid Lipid Nanoparticles (SLNs) have emerged as promising nanocarriers in cancer therapy. SLNs ...
    • Thumbnail

      Arachidyl Amido Cholanoic Acid Improves Liver Glucose and Lipid Homeostasis in Nonalcoholic Steatohepatitis Via AMPK and mTOR Regulation 

      Fernández Ramos, David; Lopitz Otsoa, Fernando; De la Cruz Villar, Laura; Bilbao García, Jon; Pagano, Martina; Mosca, Laura; Bizkarguenaga, Maider; Serrano Maciá, Marina; Azkargorta, Mikel; Iruarrizaga Lejarreta, Marta; Sot, Jesús; Tsvirkun, Darya; Van Liempd, Sebastiaan Martijn; Goñi Urcelay, Félix María; Alonso, Cristina; Martínez Chantar, María Luz; Elortza, Felix; Hayardeny, Liat; Lu, Shelly C.; Mato, José M. (Baishideng, 2020-09-14)
      BACKGROUND Arachidyl amido cholanoic acid (Aramchol) is a potent downregulator of hepatic stearoyl-CoA desaturase 1 (SCD1) protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis. ...
    • Thumbnail

      Atomistic Insights of Calmodulin Gating of Complete Ion Channels 

      Núñez, Eider; Muguruza Montero, Arantza; Villarroel Muñoz, Álvaro (MDPI, 2020-02-14)
      Intracellular calcium is essential for many physiological processes, from neuronal signaling and exocytosis to muscle contraction and bone formation. Ca<sup>2+</sup> signaling from the extracellular medium depends both on ...
    • Thumbnail

      ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP 

      Burzaco Galán, Jaione; Conde Fernández, Manuel; Parada, Luis A.; Zugaza Gurruchaga, José Luis; Dehaye, Jean-Paul; Marino Sánchez, Aida Luisa (Public Library of Science, 2013)
      In this study we have investigated the role of extracellular ATP on thrombin induced-platelet aggregation (TIPA) in washed human platelets. ATP inhibited TIPA in a dose-dependent manner and this inhibition was abolished ...
    • Thumbnail

      Bases estructurales del transporte de Mg2+ mediado por los factores homeostáticos CNNM2 y CNNM4, y de su interacción oncogénica con las fosfatasas PRL. 

      Giménez Mascarell, Paula
      El Magnesio, segundo catión intracelular más abundante, es un ión imprescindible en diversas funciones vitales como la neurotransmisión, la conductancia cardiaca o la síntesis de ATP. Este papel biológico trascendental ...
    • Thumbnail

      BFL1 Modulates Apoptosis at the Membrane Level through a Bifunctional And Multimodal Mechanism Showing Key Differences With BCLXL 

      Flores Romero, Héctor; Landeta, Olatz; Ugarte Uribe, Begoña; Cosentino, Katia; García Porras, Miguel; García-Sáez, Ana J.; Basañez, Gorka (Springer Nature, 2018-12-18)
      BFL1 is a relatively understudied member of the BCL2 protein family which has been implicated in the pathogenesis andchemoresistance of a variety of human cancers, including hematological malignancies and solid tumours. ...
    • Thumbnail

      Biomolecular Characterization of PCNA interacting proteins. 

      Martín Barros, Inés (2020-07-27)
      PCNA (Proliferating Cellular Nuclear Antigen) es la abrazadera que es desliza sobre el ADN necesaria para la replicación del ADN y la respuesta al daño. PCNA interactúa con numerosas proteínas a través de una secuencia ...