BackExaminar por departamento (eus.) "Biokimika eta biologia molekularra"
Now showing items 1-20 of 74
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A Computational Module Assembled from Different Protease Family Motifs Identifies PI PLC from Bacillus cereus as a Putative Prolyl Peptidase with a Serine Protease Scaffold
(Public Library Science, 2013-08)Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types have been systematically categorized in the MEROPS database. While a BLAST search on these proteases identifies homologous ... -
Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells
(Nature Publishing Group, 2015-09-08)Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium ... -
Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity
(Public Library of Science, 2011-02-23)Bordetella pertussis, the bacterium that causes whooping cough, secretes an adenylate cyclase toxin (ACT) that must be post-translationally palmitoylated in the bacterium cytosol to be active. The toxin targets phagocytes ... -
Advantages and Versatility of Fluorescence-Based Methodology to Characterize the Functionality of LDLR and Class Mutation Assignment
(Public Library Science, 2014-11-11)Familial hypercholesterolemia (FH) is a common autosomal codominant disease with a frequency of 1:500 individuals in its heterozygous form. The genetic basis of FH is most commonly mutations within the LDLR gene. Assessing ... -
Analysis of SUMOylated proteins using SUMO-traps
(Nature Publishing Group, 2013-04-22)SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps to capture endogenous SUMOylated proteins. Our results show ... -
Apaf-1 Inhibitors Protect from Unwanted Cell Death in In Vivo Models of Kidney Ischemia and Chemotherapy Induced Ototoxicity
(Public Library Science, 2014-10-20)Background: Excessive apoptosis induces unwanted cell death and promotes pathological conditions. Drug discovery efforts aimed at decreasing apoptotic damage initially targeted the inhibition of effector caspases. Although ... -
ATP Antagonizes Thrombin-Induced Signal Transduction through 12(S)-HETE and cAMP
(Public Library of Science, 2013)In this study we have investigated the role of extracellular ATP on thrombin induced-platelet aggregation (TIPA) in washed human platelets. ATP inhibited TIPA in a dose-dependent manner and this inhibition was abolished ... -
Bordetella pertussis-en adenilato ziklasa toxina: makrofagoetan eragindako mintz-iragazkortasun eta heriotz-zelularraren ikerketa
(2017-05-24)La toxina adenilato ciclasa(ACT) es uno de los factores de virulencia mas importantes de Bordetella pertussis, la bacteria causante de la tos ferina. ACT tiene dos actividades perjudiciales para la celul la actividad ... -
Ca2+ Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin (ACT) Endocytosis. Cell Physiology and Expression of the CD11b/CD18 Integrin Major Determinants of the Entry Route
(Public Library Publishing, 2013-09)Humans infected with Bordetella pertussis, the whooping cough bacterium, show evidences of impaired host defenses. This pathogenic bacterium produces a unique adenylate cyclase toxin (ACT) which enters human phagocytes and ... -
Calpain-Mediated Processing of Adenylate Cyclase Toxin Generates a Cytosolic Soluble Catalytically Active N-Terminal Domain
(Public Library Publishing, 2013-06)Bordetella pertussis, the whooping cough pathogen, secretes several virulence factors among which adenylate cyclase toxin (ACT) is essential for establishment of the disease in the respiratory tract. ACT weakens host ... -
Caracterización estructural de complejos ribosomales de iniciación y de pre-translocación mediante microscopía electrónica
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2012-07-04)Los ribosomas son complejos macromoleculares altamente organizados responsables de la síntesis de proteínas en todos los organismos en un proceso llamado traducción. El término traducción hace referencia a que el lenguaje ... -
Characterisation of patients with brachydactyly type E associated or not with hormonal resistance: clinical, genetic and radiological approach
(2015-11-16)La braquidatilia de tipo E es una infrecuente entidad clínica de compleja caracterización, que puede aparecer aislada o en algunas formas sindrómicas. Su infrecuencia conlleva la posibilidad de diagnosticar de forma errónea ... -
Computational and biophysical methods in characterization of protein-protein and protein-ligand interactions
(2016-02-02)Todas las moléculas - tan simples como la molécula de hidrógeno o tan extremadamente complejas como una proteína - resultan de la unión de átomos individuales. Los átomos están unidos mediante fuerzas intramoleculares ... -
Conformational Sampling and Nucleotide-Dependent Transitions of the GroEL Subunit Probed by Unbiased Molecular Dynamics Simulations
(Public Library of Science, 2011-03-10)GroEL is an ATP dependent molecular chaperone that promotes the folding of a large number of substrate proteins in E. coli. Large-scale conformational transitions occurring during the reaction cycle have been characterized ... -
Control of gene expression by modulated self-assembly
(Oxford University Press, 2011-09)Numerous transcription factors self-assemble into different order oligomeric species in a way that is actively regulated by the cell. Until now, no general functional role has been identified for this widespread process. ... -
Creating stable Floquet–Weyl semimetals by laser-driving of 3D Dirac materials
(Nature Publishing, 2017-01-17)Tuning and stabilizing topological states, such as Weyl semimetals, Dirac semimetals or topological insulators, is emerging as one of the major topics in materials science. Periodic driving of many-body systems offers a ... -
Degradación de alcanos mediante Alcanivorax venutensis inmovilizada en hidrogeles adhesivos y biodegradables
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2011-01-27)El petróleo y sus derivados son los mayores contaminantes del medio marino. Además puntualmente se producen grandes vertidos accidentales como el que en Noviembre de 2002 produjo un desastre ecológico sin precedentes en ... -
Delivery of small nucleic acids by conjugation to carbohydrates and lipids as novel research and therapeutic tools
(2014-03-12)Small nucleic acids present a great potential to silence specific genes or inhibit the biological activity of specific proteins. Unfortunately, their poor cellular accessibility presents a big challenge for developing an ... -
Desarrollo de líneas celulares competentes para la replicación de priones humanos mediante la reprogramación de fibroblastos de pacientes genéticamente susceptibles
(2016-07-05)Las enfermedades espongiformes transmisibles (EETs) o enfermedades priónicas son un grupo de enfermedades neurodegenerativas mortales que afectan a seres humanos y animales, son producidas por el mal plegamiento de la ... -
Desarrollo de un nuevo soporte polímerico con componentes de matriz extracelular neural para su aplicación en la diferenciación "in vitro" de distintas células primarias
(2012-07-11)Este trabajo de Tesis Doctoral presenta el desarrollo, la caracterización y la validación de un nuevo soporte polimérico capaz de inducir la diferenciación neuronal in vitro, combinando técnicas de fotolitografía y de ...