Show simple item record

dc.contributor.authorVarona Gutiérrez, Adolfo
dc.contributor.authorBlanco Criado, Lorena
dc.contributor.authorPérez Urcelai, Itxaro
dc.contributor.authorGil Goicouría, Francisco Javier ORCID
dc.contributor.authorIrazusta Astiazaran, Jon ORCID
dc.contributor.authorLópez Fernández de Villaverde, José Ignacio ORCID
dc.contributor.authorCandenas, M. Luz
dc.contributor.authorPinto, Francisco M.
dc.contributor.authorLarrinaga Embeita, Gorka ORCID
dc.date.accessioned2014-04-01T14:19:21Z
dc.date.available2014-04-01T14:19:21Z
dc.date.issued2010-05
dc.identifier.citationBmc Cancer 10 : (2010) // Article n. 193es
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/10810/11897
dc.description.abstractBackground: Cell-surface glycoproteins play critical roles in cell-to-cell recognition, signal transduction and regulation, thus being crucial in cell proliferation and cancer etiogenesis and development. DPP IV and NEP are ubiquitous glycopeptidases closely linked to tumor pathogenesis and development, and they are used as markers in some cancers. In the present study, the activity and protein and mRNA expression of these glycoproteins were analysed in a subset of clear-cell (CCRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytomas (RO). Methods: Peptidase activities were measured by conventional enzymatic assays with fluorogen-derived substrates. Gene expression was quantitatively determined by qRT-PCR and membrane-bound protein expression and distribution analysis was performed by specific immunostaining. Results: The activity of both glycoproteins was sharply decreased in the three histological types of renal tumors. Protein and mRNA expression was strongly downregulated in tumors from distal nephron (ChRCC and RO). Moreover, soluble DPP IV activity positively correlated with the aggressiveness of CCRCCs (higher activities in high grade tumors). Conclusions: These results support the pivotal role for DPP IV and NEP in the malignant transformation pathways and point to these peptidases as potential diagnostic markers.es
dc.language.isoenges
dc.publisherBioMed Centrales
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectdipeptidyl peptidase IVes
dc.subjectreal time PCRes
dc.subjectcell carcinomaes
dc.subjectdifferential diagnosises
dc.subjectneutral endopeptidasees
dc.subjecttachykinin receptorses
dc.subjectextracellular matrix;es
dc.subjectCD26es
dc.subjectCD10es
dc.subjectprogressiones
dc.titleExpression and activity profiles of DPP IV/CD26 and NEP/CD10 glycoproteins in the human renal cancer are tumor-type dependentes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2010 Varona et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://www.biomedcentral.com/1471-2407/10/193es
dc.identifier.doi10.1186/1471-2407-10-193
dc.departamentoesEnfermeríaes_ES
dc.departamentoesFisiologíaes_ES
dc.departamentoeuErizaintzaes_ES
dc.departamentoeuFisiologiaes_ES
dc.subject.categoriaGENETICS AND HEREDITY
dc.subject.categoriaONCOLOGY


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record