dc.contributor.advisor | Iglesias Ara, Ainhoa | es |
dc.contributor.author | Zuazo Gaztelu, Iratxe | es |
dc.contributor.other | F. CIENCIA Y TECNOLOGIA | es |
dc.contributor.other | ZIENTZIA ETA TEKNOLOGIA F. | eu |
dc.date.accessioned | 2015-04-01T11:16:23Z | |
dc.date.available | 2015-04-01T11:16:23Z | |
dc.date.issued | 2015-04-01 | |
dc.identifier.uri | http://hdl.handle.net/10810/14847 | |
dc.description.abstract | E2F1 and E2F2 transcription factors have an important role during the regulation of cell cycle. In experiments done with E2F1/E2F2 knockout mice, it has been described that bone-marrow-derived macrophages (BMDM) undergo an early rapid proliferation event related to DNA hyper-replication. As a consequence, DNA damage response (DDR) pathway is triggered and E2F1/E2F2 knockout macrophages enter premature senescence related to G2/M phase arrest. The exact mechanism trough which DNA hyper-replication leads to DDR in absence of E2F1 and E2F2 remains undiscovered. To determine whether the ATR/ATM pathway, the master regulator of G2/M checkpoint, might be the surveillance mechanism in order to regulate uncontrolled proliferation in the DKO model, we monitored and analysis biochemical properties of BMDM cultures in the presence of caffeine, a potent inhibitor of ATM/ATR activity. Our results show that the addition of caffeine abolishes premature senescence in DKO BMDM, stimulates γ-H2AX accumulation and decreases Mcm2 expression. | es |
dc.language.iso | eng | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | E2F1/E2F2-/- | es |
dc.subject | caffeine | es |
dc.subject | ATM/ATR | es |
dc.title | Analysing the contribution of ATM/ATR pathway activation in establishing the premature senescence of E2F1/E2F2-/- bone-marrow-derived macrophages | es |
dc.type | info:eu-repo/semantics/bachelorThesis | es |
dc.date.updated | 2014-06-20T11:04:43Z | es |
dc.language.rfc3066 | en | es |
dc.rights.holder | © 2014, EL AUTOR | es |
dc.contributor.degree | Grado en Bioquímica y Biología Molecular | es |
dc.contributor.degree | Biokimikako eta Biologia Molekularreko Gradua | es |
dc.identifier.gaurregister | 51830-631146-10 | es |
dc.identifier.gaurassign | 8206-631146 | es |