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dc.contributor.advisorZubiaga Elordieta, Ana Maríaes
dc.contributor.authorDehesa Etxebeste, Martxel Pedroes
dc.contributor.otherF. CIENCIA Y TECNOLOGIAes
dc.contributor.otherZIENTZIA ETA TEKNOLOGIA F.
dc.date.accessioned2015-04-01T13:48:29Z
dc.date.available2015-04-01T13:48:29Z
dc.date.issued2014-02-11T15:56:11Zes
dc.identifier.urihttp://hdl.handle.net/10810/14909
dc.description.abstractIn this work we wanted to study the mechanism of E2F2-mediated repression. Our hypothesis is that E2F2 activates the expression of one or more E2F members of the “repressor” subset of the family through the E2F motifs present in their promoters, and those repressor E2F(s) would subsequently repress the target promoters. To address this hypothesis, we focused on E2F7. E2F7 is a repressor that lacks the Rb binding domain, and associates with DNA through E2F binding sites (de Bruin et al., 2003). Furthermore, E2F7 itself is also regulated by E2F motifs on its own promoter, and it has been shown to repress DNA metabolism and replication genes in late S-phase (de Bruin et al., 2003; Westendorp et al., 2012). E2F7, together with E2F8 has been found to form heterodimers, being critical on cell proliferation and development, and both seem to have similar functions (Li et al., 2008). Preliminary results from Zubiaga’s group have indicated that E2F2 activates E2F7 transcription in U2OS cells, suggesting that E2F2’s repressor function could be mediated by E2F7. For this purpose, we focused on studying E2F7’s role on the target genes previously known to be repressed by E2F2: Chk1 and Mcm5. The specific aims for this work were the following: - Confirm that E2F2 induces E2F7 in HEK-293T cells - Assess whether E2F7 acts as a transcriptional repressor on E2F sites - Evaluate the role of E2F7 on E2F2-mediated transcriptional repression of Chk1 and Mcm5.es
dc.language.isoenges
dc.rightsinfo:eu-repo/Semantics/openAccesses
dc.subjectE2F2es
dc.subjectRepressiones
dc.titleMechanisms of E2F2-mediated transcriptional repressiones
dc.typeinfo:eu-repo/semantics/bachelorThesises
dc.date.updated2014-02-11T12:06:39Zes
dc.language.rfc3066enes
dc.rights.holder© 2014, EL AUTORes
dc.contributor.degreeGrado en Bioquímica y Biología Moleculares
dc.contributor.degreeBiokimikako eta Biologia Molekuarreko Gradua
dc.identifier.gaurassign8117-602730es


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