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dc.contributor.authorCanduela Pérez, Miren Josune
dc.contributor.authorMendizabal Zubiaga, Juan Luis ORCID
dc.contributor.authorPuente Bustinza, Nagore
dc.contributor.authorReguero Acebal, Leire
dc.contributor.authorElezgarai Gabantxo, Izaskun ORCID
dc.contributor.authorRamos Uriarte, Almudena
dc.contributor.authorGerrikagoitia Marina, Inmaculada ORCID
dc.contributor.authorGrandes Moreno, Pedro Rolando ORCID
dc.date.accessioned2016-01-22T15:38:36Z
dc.date.available2016-01-22T15:38:36Z
dc.date.issued2015-03-16
dc.identifier.citationPLoS ONE 10(3) : (2015) // e0119401es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/16798
dc.description.abstractWe have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (similar to 60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present.es
dc.description.sponsorshipP. Grandes' laboratory is provided by Ministerio de Economia y Competitividad (BFU2012-33334); Basque Government grant IT764-13; University of the Basque Country (UFI11/41) and by Red de Trastornos Adictivos, RETICS, Instituto de Salud Carlos III, grant number: RD07/0001/2001, RD12/0028/0004.es
dc.language.isoenges
dc.publisherPublic Library Sciencees
dc.relationinfo:eu-repo/grantAgreement/MINECO/BFU2012-33334
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectlong term depressiones
dc.subjectTRPV1 channelses
dc.subjectimmunohistochemical localizationes
dc.subjectendocannabinoid systemes
dc.subjectcannabinoid receptores
dc.subjectsynaptic plasticityes
dc.subjectbraines
dc.subjectmicees
dc.subjectexpressiones
dc.subjecthypocampuses
dc.titleVisualization by High Resolution Immunoelectron Microscopy of the Transient Receptor Potential Vanilloid-1 at Inhibitory Synapses of the Mouse Dentate Gyruses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2015 Canduela et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedes
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119401es
dc.identifier.doi10.1371/journal.pone.0119401
dc.departamentoesNeurocienciases_ES
dc.departamentoeuNeurozientziakes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaMEDICINE
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY


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