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dc.contributor.authorAlbaina Vivanco, Aitor
dc.contributor.authorAguirre Rodrigo, Mikel
dc.contributor.authorAbad García, David
dc.contributor.authorSantos, María
dc.contributor.authorEstomba Recalde, Miren Andone
dc.date.accessioned2016-05-12T13:10:05Z
dc.date.available2016-05-12T13:10:05Z
dc.date.issued2016-03
dc.identifier.citationEcology and Evolution 6(6) : 1809-1824 (2016)es
dc.identifier.issn2045-7758
dc.identifier.urihttp://hdl.handle.net/10810/18240
dc.description.abstractThe potential of the 18S rRNA V9 metabarcoding approach for diet assessment was explored using MiSeq paired-end (PE; 2 9 150 bp) technology. To critically evaluate the method's performance with degraded/digested DNA, the diets of two zooplanktivorous fish species from the Bay of Biscay, European sardine (Sardina pilchardus) and European sprat (Sprattus sprattus), were analysed. The taxonomic resolution and quantitative potential of the 18S V9 metabarcoding was first assessed both in silico and with mock and field plankton samples. Our method was capable of discriminating species within the reference database in a reliable way providing there was at least one variable position in the 18S V9 region. Furthermore, it successfully discriminated diet between both fish species, including habitat and diel differences among sardines, overcoming some of the limitations of traditional visual-based diet analysis methods. The high sensitivity and semi-quantitative nature of the 18S V9 metabarcoding approach was supported by both visual microscopy and qPCR-based results. This molecular approach provides an alternative cost and time effective tool for food-web analysis.es
dc.description.sponsorshipThe sampling was financially supported by the projects ECOGENBAY (MICINN CTM2009-13570-C02-02), funded by the Government of Spain, and the project "Bay of Biscay anchovy biomass estimates for year 2010", funded by the Basque Government and by the European Commission within the frame of the National Sampling Programme (DCF No. 665/2008 14 July 2008). The General Secretariat of Sea also collaborated providing the R/V Emma Bardan. AA and DA work was supported by a contract with the Euskampus Foundation (Euskampus Fundazioa) and a doctoral fellowship of the Basque Government (UPV/EHU "ZabaldUz" program), respectively. This study was funded by a Basque Government grant to "Grupo de Investigacion consolidado del sistema universitario vasco, IT558-10".es
dc.language.isoenges
dc.publisherWiley Blackwelles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subject18S rRNA V9es
dc.subjectdiet analysises
dc.subjectmetabarcodinges
dc.subjectMiSeq paired-end technologyes
dc.subjectplanktones
dc.subjectsardina pilcharduses
dc.subjectsprattus sprattuses
dc.subjectreal-time PCRes
dc.subjecthigh throughputes
dc.subjectcopy munberes
dc.subjectnorth-seaes
dc.subjectDNAes
dc.subjectbiodiversityes
dc.subjectcommunitieses
dc.subjectdiversityes
dc.subjectcopepodses
dc.title18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish specieses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder2016 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/ece3.1986/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+14th+May+11:00-14:00+BST+/+06:00-09:00+EDT+/+18:00-21:00+SGT+for+essential+maintenance.Apologies+for+the+inconvenience.es
dc.identifier.doi10.1002/ece3.1986
dc.departamentoesGenética, antropología física y fisiología animales_ES
dc.departamentoeuGenetika,antropologia fisikoa eta animalien fisiologiaes_ES


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