Quantitative real-time PCR data analysis with R
Machado de Souza, Ignacio Nicolás
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This dissertation covers an introduction to the field of qPCR data analysis using the state-of-the-art R programming language. qPCR analyses genomic data based on the DNA replication. After showing a set of basic biological and statistics concepts, the roots of qPCR technology, together with its principal data analysis modelisations (visualisation, filtering, clustering, ...) are shown. A web application has been developed to ease and extend qPCR data analysis functionalities to other areas such as biology or forensics.