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dc.contributor.authorNavarro, Virginia
dc.contributor.authorPortillo Baquedano, María Puy ORCID
dc.contributor.authorMargotat, Alain
dc.contributor.authorLandrier, Jean-François
dc.contributor.authorMacarulla Arenaza, María Teresa
dc.contributor.authorLairon, Denis
dc.contributor.authorMartin, Jean-Charles
dc.date.accessioned2011-05-20T16:22:55Z
dc.date.available2011-05-20T16:22:55Z
dc.date.issued2009-08-28
dc.identifier.citationBritish Journal of Nutrition 102(4) : 537-545 (2009)es
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10810/2566
dc.description.abstractIn mice, hepatic functions can be greatly affected by dietary trans-10, cis-12-conjugated linoleic acid (CLA). However, this phenomenon has been less documented in hamsters. In the present study, male hamsters were fed two doses of the trans-10, cis-12-CLA (0·5 and 1 %, w/w diet) or linoleic acid (0·5 %) for 6 weeks. The effects on the liver were examined by measuring the expression of thirty-six genes representing key metabolic pathways. CLA-responsive genes and their relationships with physiological outcomes were examined by a multivariate analysis procedure. Compared with control hamsters, those receiving either 0·5 or 1 % CLA exhibited similar fat loss (15–24 %; P ≤ 0·05) and liver enlargement (21–28 %; P ≤ 0·05), with no signs of steatosis. We also observed a dose-dependent increase in the transcription of genes involved in lipid breakdown and lipid harvesting from blood, and in genes related to the oxidative stress and inflammatory responses. These responsive genes varied in parallel with cell membrane lipids (R2 0·31–0·42) and to a lesser extent with liver enlargement (R2 0·22) (all P < 0·05). We conclude that in hamsters, liver enlargement induced by trans-10, cis-12-CLA is accompanied by an increased metabolic potential to process fatty acids from mobilised adipose stores. This elevated metabolic activity, comprised of anabolic pathways and their catabolic counterparts, can trigger inflammation and the oxidant stress defence pathways in a dose-dependent manner. These results provide novel insights into the mechanisms by which trans-10, cis-12-CLA affects pathways related to liver function.es
dc.description.sponsorshipsupported by grants from the Ministerio de Ciencia y Tecnologia (BFI2002-00273) and the University of Pais Vasco (00 101. 125-15 340/2003). V. N. has a doctoral fellowship from the Spanish Government (Ministerio de Educacion y Ciencia). Part of the experiment was funded by the Institut National de la Recherche Agronomique (INRA) and by the Institut National de la Recherche Medicale (INSERM), Francees
dc.language.isoenges
dc.publisherCambridge University Presses
dc.relationinfo:eu-repo/grantAgreement/MCYT/BFI2002-00273
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectconjugated linoleic acides
dc.subjectliver gene expressiones
dc.subjecthamsterses
dc.subjectlipid metabolismes
dc.subjectpartial least square regressiones
dc.titleA multi-gene analysis strategy identifies metabolic pathways targeted by trans-10, cis-12-conjugated linoleic acid in the liver of hamsterses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holderCopyright © The Authors 2009es
dc.relation.publisherversionhttp://journals.cambridge.org/abstract_S0007114509231734es
dc.identifier.doi10.1017/S0007114509231734
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES
dc.subject.categoriaMEDICINE
dc.subject.categoriaNUTRITION AND DIETETICS


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