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dc.contributor.authorDíaz Núñez, María
dc.contributor.authorDíez Torre, Alejandro
dc.contributor.authorDe Wever, Olivier
dc.contributor.authorAndrade Pocino, Ricardo
dc.contributor.authorArluzea de Jauregizar, Jon Andoni
dc.contributor.authorSilió López, Margarita Rosa
dc.contributor.authorAréchaga Martínez, Juan Miguel
dc.date.accessioned2019-04-10T10:39:49Z
dc.date.available2019-04-10T10:39:49Z
dc.date.issued2016-08-22
dc.identifier.citationBMC Cancer 16 : (2016) // Article ID 667es_ES
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/10810/32406
dc.description.abstractBackground: Histone deacetylase inhibitors (HDACi) exert multiple cytotoxic actions on cancer cells. Currently, different synthetic HDACi are in clinical use or clinical trials; nevertheless, since both pro-invasive and anti-invasive activities have been described, there is some controversy about the effect of HDACi on melanoma cells. Methods: Matrigel and Collagen invasion assays were performed to evaluate the effect of several HDACi (Butyrate, Trichostatin A, Valproic acid and Vorinostat) on two human melanoma cell line invasion (A375 and HT-144). The expression of N- and E-Cadherin and the activity of the RhoA GTPase were analyzed to elucidate the mechanisms involved in the HDACi activity. Results: HDACi showed a pro-invasive effect on melanoma cells in vitro. This effect was accompanied by an up-regulation of N-cadherin expression and an inhibition of RhoA activity. Moreover, the down-regulation of N-cadherin through blocking antibodies or siRNA abrogated the pro-invasive effect of the HDACi and, additionally, the inhibition of the Rho/ROCK pathway led to an increase of melanoma cell invasion similar to that observed with the HDACi treatments. Conclusion: These results suggest a role of N-cadherin and RhoA in HDACi induced invasion and call into question the suitability of some HDACi as antitumor agents for melanoma patients.es_ES
dc.description.sponsorshipResearch grants from the Spanish Ministry of Economy and Competitiveness (SAF2012-39773), the regional Basque Government Research Groups Program (T560-10) and the SAIOTEK Program of the Basque Science, Technology & Innovation net (SPRI) to J. Arechaga supported this work. M. Diaz-Nunez held a fellowship from the University of the Basque Country and, later on, from the Jesus Gangoiti Barrera Foundation of Bilbao.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/SAF2012-39773es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjecthistone deacetylase inhibitorses_ES
dc.subjectHDACies_ES
dc.subjectmelanomaes_ES
dc.subjectcell invasiones_ES
dc.subjectN-cadherines_ES
dc.subjectRhoaes_ES
dc.subjectepithelial-mesenchymal transitionses_ES
dc.subjectcancer-cellses_ES
dc.subjectbreast-canceres_ES
dc.subjectcarcinoma metastasises_ES
dc.subjectpancreatic-canceres_ES
dc.subjectgene-expressiones_ES
dc.subjectHDAC inhibitorses_ES
dc.subjectvalproices_ES
dc.subjectlung-canceres_ES
dc.subjectstem-cellses_ES
dc.titleHistone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2693-3es_ES
dc.identifier.doi10.1186/s12885-016-2693-3
dc.departamentoesBiología celular e histologíaes_ES
dc.departamentoeuZelulen biologia eta histologiaes_ES


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.