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dc.contributor.authorErcibengoa, María
dc.contributor.authorAlonso, Marta
dc.contributor.authorVicente Anza, Diego
dc.contributor.authorMorales Areizaga, María
dc.contributor.authorGarcía López, Ernesto Ángel
dc.contributor.authorMarimón Ortiz de Zarate, José María
dc.date.accessioned2019-04-30T10:07:25Z
dc.date.available2019-04-30T10:07:25Z
dc.date.issued2019-02-12
dc.identifier.citationPlos One 14(2) : (2019) // Article ID e0212022es_ES
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/32590
dc.description.abstractNowadays, more than 95 different Streptococcus pneumoniae serotypes are known, being less than one third responsible for the majority of severe pneumococcal infections. After the introduction of conjugate vaccines, a change in the epidemiology of the serotypes causing invasive pneumococcal disease has been observed making the surveillance of circulating serotypes especially relevant. Some recent studies have used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology to identify the most frequent pneumococcal serotypes that cause invasive disease. The objectives of this study were to evaluate the efficacy of previously described discriminatory peaks determined by MALDI-TOF MS for the identification of serotypes 6B, 19F, 19A and 35B using reference and clinical isolates and to try to identify other discriminatory peaks for serotypes 11A, 19F and 19A using transformed pneumococcal strains. Most of the proposed peaks defined in the literature for the identification of serotypes 6B, 19F, 19A, 35B were not found in the spectra of the 10 reference isolates nor in those of the 60 clinical isolates tested corresponding to these four serotypes. The analysis and comparison of the mass spectra of genetically modified pneumococci (transformed strains) did not allow the establishment of new discriminatory peaks for serotypes 11A, 19F, and 19A. MALDI-TOF MS in the usual range of 2,000 to 20,000 m/z did not prove to be a valid technique for direct S. pneumoniae serotyping.es_ES
dc.description.sponsorshipThis work was funded in part by a grant of the Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias (CIBERES), CB06/06/0056 and CB06/06/0003. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscriptes_ES
dc.language.isoenges_ES
dc.publisherPublic Library Sciencees_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectdesorption ionization-timees_ES
dc.subjectmultiplex PCR assayes_ES
dc.subjectstaphylococcus-aureuses_ES
dc.subjectmass-spectrometryes_ES
dc.subjectcapsular polysaccharidees_ES
dc.subjectculture-conditionses_ES
dc.subjectidentificationes_ES
dc.subjectdifferentiationes_ES
dc.subjectimmunoassayes_ES
dc.subjectpneumococcies_ES
dc.titleUtility of MALDI-TOF MS as a New Tool for Streptococcus Pneumoniae Serotypinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderAttribution 4.0 International (CC BY 4.0) You are free to: copy and redistribute the material in any medium or format . Remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms.es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212022es_ES
dc.identifier.doi10.1371/journal.pone.0212022
dc.departamentoesMedicina preventiva y salud públicaes_ES
dc.departamentoeuPrebentzio medikuntza eta osasun publikoaes_ES


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Attribution 4.0 International (CC BY 4.0) You are free to:
copy and redistribute the material in any medium or format . Remix, transform, and build upon the material  for any purpose, even commercially.  The licensor cannot revoke these freedoms as long as you follow the license terms.
Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0) You are free to: copy and redistribute the material in any medium or format . Remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms.