dc.contributor.author | Azuaje Hualde, Enrique | |
dc.contributor.author | Arroyo Jiménez, Susana | |
dc.contributor.author | Garai Ibabe, Gaizka | |
dc.contributor.author | Benito López, Fernando | |
dc.contributor.author | Martínez de Pancorbo Gómez, María de los Angeles | |
dc.date.accessioned | 2020-06-01T10:31:47Z | |
dc.date.available | 2020-06-01T10:31:47Z | |
dc.date.issued | 2020-05-07 | |
dc.identifier.citation | Analytica Chimica Acta 1123 : 1-8 (2020) | es_ES |
dc.identifier.issn | 0003-2670 | |
dc.identifier.uri | http://hdl.handle.net/10810/43659 | |
dc.description.abstract | Nowadays, there are strong efforts in developing new technology for rapid detection of specific DNA sequences for environmental monitoring, forensic analysis and rapid biomedical diagnosis applications. That is where microfluidic paper-based analytical devices are positioned as suitable platforms for the development of point of care analytical devices, due to their simple fabrication protocols, ease of use and low cost. Herein, a methodology for in situ single strand DNA detection by using a colorimetric assay based on the formation of a DNAzyme within a paper substrate was developed. A DNAzyme that could only be formed in the presence of a specific sequence of the Y human amelogenin gene was designed. The performance of the DNAzyme was followed colorimetrically first in solution and then in paper substrates. The reaction was found to be specific to the Y fragment selected as analyte. The DNAzyme reaction on paper enabled the unequivocal colorimetric identification of the Y single strand DNA fragment both qualitatively, with the naked eye (143 ng), and quantitatively by image analysis (45.7 ng). As a proof of concept, a microfluidic paper-based device, pre-loaded with all DNAzyme reagents, was characterized and implemented for the simultaneous detection of X and Y single strand DNA fragments. | es_ES |
dc.description.sponsorship | This work was supported by Gobierno de España, Ministerio de Economía y Competitividad, with Grant No. BIO2016-80417-P; funding from Basque Government (Grant: IT1271-19) and European Union funds: DNASURF (H2020-MSCA-RISE-778001). E.A.-H. acknowledges funding from the Basque Government, Department of Education, for predoctoral fellowship 2016. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/778001 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/BIO2016-80417-P | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | |
dc.subject | DNAzyme, amelogenin, paper substrate, gene detection, colorimetric, paper based microfluidics | es_ES |
dc.subject | DNAzyme | es_ES |
dc.subject | amelogenin | es_ES |
dc.subject | paper substrate | es_ES |
dc.subject | gene detection | es_ES |
dc.subject | colorimetric | es_ES |
dc.subject | paper based microfluidics | es_ES |
dc.title | Naked Eye Y Amelogenin Gene Fragment Detection Using DNAzymes on a Paper-based Device | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2020 Elsevier B.V. Atribución-NoComercial-SinDerivadas 4.0 | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0003267020305237?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.aca.2020.05.010 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Química analítica | es_ES |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Kimika analitikoa | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |