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dc.contributor.authorAzuaje Hualde, Enrique ORCID
dc.contributor.authorArroyo Jiménez, Susana
dc.contributor.authorGarai Ibabe, Gaizka
dc.contributor.authorBenito López, Fernando ORCID
dc.contributor.authorMartínez de Pancorbo Gómez, María de los Angeles ORCID
dc.date.accessioned2020-06-01T10:31:47Z
dc.date.available2020-06-01T10:31:47Z
dc.date.issued2020-05-07
dc.identifier.citationAnalytica Chimica Acta 1123 : 1-8 (2020)es_ES
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/10810/43659
dc.description.abstractNowadays, there are strong efforts in developing new technology for rapid detection of specific DNA sequences for environmental monitoring, forensic analysis and rapid biomedical diagnosis applications. That is where microfluidic paper-based analytical devices are positioned as suitable platforms for the development of point of care analytical devices, due to their simple fabrication protocols, ease of use and low cost. Herein, a methodology for in situ single strand DNA detection by using a colorimetric assay based on the formation of a DNAzyme within a paper substrate was developed. A DNAzyme that could only be formed in the presence of a specific sequence of the Y human amelogenin gene was designed. The performance of the DNAzyme was followed colorimetrically first in solution and then in paper substrates. The reaction was found to be specific to the Y fragment selected as analyte. The DNAzyme reaction on paper enabled the unequivocal colorimetric identification of the Y single strand DNA fragment both qualitatively, with the naked eye (143 ng), and quantitatively by image analysis (45.7 ng). As a proof of concept, a microfluidic paper-based device, pre-loaded with all DNAzyme reagents, was characterized and implemented for the simultaneous detection of X and Y single strand DNA fragments.es_ES
dc.description.sponsorshipThis work was supported by Gobierno de España, Ministerio de Economía y Competitividad, with Grant No. BIO2016-80417-P; funding from Basque Government (Grant: IT1271-19) and European Union funds: DNASURF (H2020-MSCA-RISE-778001). E.A.-H. acknowledges funding from the Basque Government, Department of Education, for predoctoral fellowship 2016.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/778001es_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/BIO2016-80417-Pes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectDNAzyme, amelogenin, paper substrate, gene detection, colorimetric, paper based microfluidicses_ES
dc.subjectDNAzymees_ES
dc.subjectamelogenines_ES
dc.subjectpaper substratees_ES
dc.subjectgene detectiones_ES
dc.subjectcolorimetrices_ES
dc.subjectpaper based microfluidicses_ES
dc.titleNaked Eye Y Amelogenin Gene Fragment Detection Using DNAzymes on a Paper-based Devicees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holder© 2020 Elsevier B.V. Atribución-NoComercial-SinDerivadas 4.0es_ES
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0003267020305237?via%3Dihubes_ES
dc.identifier.doi10.1016/j.aca.2020.05.010
dc.contributor.funderEuropean Commission
dc.departamentoesQuímica analíticaes_ES
dc.departamentoesZoología y biología celular animales_ES
dc.departamentoeuKimika analitikoaes_ES
dc.departamentoeuZoologia eta animalia zelulen biologiaes_ES


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© 2020 Elsevier B.V. Atribución-NoComercial-SinDerivadas 4.0
Except where otherwise noted, this item's license is described as © 2020 Elsevier B.V. Atribución-NoComercial-SinDerivadas 4.0