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Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

dc.contributor.authorQuerido, Jailson F. B.
dc.contributor.authorAgirre, Jon
dc.contributor.authorMartí, Gerardo A.
dc.contributor.authorGuerín Aguilar, Diego Marcelo A. ORCID
dc.contributor.authorSilva, Marcelo Sousa
dc.date.accessioned2014-02-05T17:35:52Z
dc.date.available2014-02-05T17:35:52Z
dc.date.issued2013
dc.identifier.citationBioMed Research International 2013 : (2013) // Article ID 218593es
dc.identifier.issn2314-6133
dc.identifier.urihttp://hdl.handle.net/10810/11360
dc.description.abstractDicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.es
dc.description.sponsorshipon Agirre was supported by a UPV/EHU contract (UPV-IT-461-07). Gerardo A. Marti is a researcher of the CONICET, Argentina. Diego M. A. Guerin is researcher of the Fundacion Biofisica Bizkaia and was partially supported by Grants SAIOTEK, Gobierno Vasco (GV), and MICINN, Spain. This work was partially supported by Accion Especial AE-2009-1-21, from the GV, Spain, and CYTED 209RT0364 action (RedTrV: http://www.redtrv.org/), both granted to Diego M. A. Guerin.es
dc.language.isoenges
dc.publisherHindawi Publishing Corporationes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectTriatoma viruses
dc.subjectinfestans hemipteraes
dc.subjectreduviidaees
dc.titleMolecular Techniques for Dicistrovirus Detection without RNA Extraction or Purificationes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2013 Jailson F. B. Querido et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://www.hindawi.com/journals/bmri/2013/218593/es
dc.identifier.doi10.1155/2013/218593
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES


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