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Nitrogen Source and External Medium pH Interaction Differentially Affects Root and Shoot Metabolism in Arabidopsis
dc.contributor.author | Sarasqueta Gómez, Asier | |
dc.contributor.author | González Moro, María Begoña | |
dc.contributor.author | González Murua, María del Carmen Begoña | |
dc.contributor.author | Marino Bilbao, Daniel | |
dc.date.accessioned | 2016-05-18T15:18:45Z | |
dc.date.available | 2016-05-18T15:18:45Z | |
dc.date.issued | 2016-02-01 | |
dc.identifier.citation | Frontiers in Plant Science 7 : (2016) // Article ID 29 | es |
dc.identifier.issn | 1664-462X | |
dc.identifier.uri | http://hdl.handle.net/10810/18278 | |
dc.description.abstract | Ammonium nutrition often represents an important growth-limiting stress in plants. Some of the symptoms that plants present under ammonium nutrition have been associated with pH deregulation, in fact external medium pH control is known to improve plants ammonium tolerance. However, the way plant cell metabolism adjusts to these changes is not completely understood. Thus, in this work we focused on how Arabidopsis thaliana shoot and root respond to different nutritional regimes by varying the nitrogen source (NO3 and NH4), concentration (2 and 10 mM) and pH of the external medium (5.7 and 6.7) to gain a deeper understanding of cell metabolic adaptation upon altering these environmental factors. The results obtained evidence changes in the response of ammonium assimilation machinery and of the anaplerotic enzymes associated to Tricarboxylic Acids (TCA) cycle in function of the plant organ, the nitrogen source and the degree of ammonium stress. A greater stress severity at pH 5.7 was related to NH4 accumulation; this could not be circumvented in spite of the stimulation of glutamine synthetase, glutamate dehydrogenase, and TCA cycle anaplerotic enzymes. Moreover, this study suggests specific functions for different gin and gdh isoforms based on the nutritional regime. Overall, NH4 accumulation triggering ammonium stress appears to bear no relation to nitrogen assimilation impairment. | es |
dc.description.sponsorship | AS is holder of a Ph.D. grant from the Basque Government. This research was financially supported by the Basque Government (IT932-16), the University of the Basque Country (EHUA14/14), the Spanish Ministry of Economy and Competitiveness (BIO2014-56271-R co-funded by FEDER) and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement number 334019. We are also grateful to Dr. J. F. Moran and Dr. K. A. Roubelakis-Angelakis for lending CS and GDH antibodies, respectively. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation | info:eu-repo/grant/Agreement/EC/FP7/334019 | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | ammonium | es |
dc.subject | Arabidopsis thaliana | es |
dc.subject | glutamate dehydrogenase | es |
dc.subject | glutamine synthetase | es |
dc.subject | nitrate | es |
dc.subject | nitrogen metabolism | es |
dc.subject | pH | es |
dc.subject | TCA cycle | es |
dc.subject | cytisikic glutamine-sinthetase | es |
dc.subject | alleviates ammonium toxixity | es |
dc.subject | menbrane H+-atpase | es |
dc.subject | isocitrate dehydrogenase | es |
dc.subject | crucial role | es |
dc.subject | phosphoenolpiruvate carboxylase | es |
dc.subject | plant-growth | es |
dc.subject | zea-mays | es |
dc.subject | key role | es |
dc.subject | nutrition | es |
dc.title | Nitrogen Source and External Medium pH Interaction Differentially Affects Root and Shoot Metabolism in Arabidopsis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | ©2016Sarasketa,González-Moro,González-MuruaandMarino.This isanopen-accessarticledistributedunderthetermsofthe CreativeCommons AttributionLicense(CCBY).Theuse,distributionorreproductioninotherforums ispermitted,providedtheoriginalauthor(s)orlicensorarecreditedandthatthe originalpublicationinthisjournaliscited,inaccordance withacceptedacademic practice.Nouse,distributionorreproductionispermitted whichdoesnotcomply withtheseterms. FrontiersinPlantScience|www. | es |
dc.relation.publisherversion | http://journal.frontiersin.org/article/10.3389/fpls.2016.00029/full#h1 | es |
dc.identifier.doi | 10.3389/fpls.2016.00029 | |
dc.departamentoes | Biología vegetal y ecología | es_ES |
dc.departamentoeu | Landaren biologia eta ekologia | es_ES |
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