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Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition
dc.contributor.author | Goikuria Iriondo, Haize | |
dc.contributor.author | Freijo, María del Mar | |
dc.contributor.author | Vega Manrique, Reyes | |
dc.contributor.author | Sastre, María | |
dc.contributor.author | Elizagaray, Elena | |
dc.contributor.author | Lorenzo, Ana | |
dc.contributor.author | Vandenbroeck, Koen | |
dc.contributor.author | Alloza Moral, Iraide | |
dc.date.accessioned | 2019-03-12T13:51:09Z | |
dc.date.available | 2019-03-12T13:51:09Z | |
dc.date.issued | 2018-03 | |
dc.identifier.citation | Cells 7(3) : (2018) // Article ID 23 | es_ES |
dc.identifier.issn | 2073-4409 | |
dc.identifier.uri | http://hdl.handle.net/10810/31986 | |
dc.description.abstract | Vascular smooth muscle cells (VSMCs) are central players in carotid atherosclerosis plaque development. Although the precise mechanisms involved in plaque destabilization are not completely understood, it is known that VSMC proliferation and migration participate in plaque stabilization. In this study, we analyzed expression patterns of genes involved in carotid atherosclerosis development (e.g., transcription factors of regulation of SMC genes) of VSMCs located inside or outside the plaque lesion that may give clues about changes in phenotypic plasticity during atherosclerosis. VSMCs were isolated from 39 carotid plaques extracted from symptomatic and asymptomatic patients by endarterectomy. Specific biomarker expression, related with VSMC phenotype, was analyzed by qPCR, western immunoblot, and confocal microscopy. MYH11, CNN1, SRF, MKL2, and CALD1 were significantly underexpressed in VSMCs from plaques compared with VSMCs from a macroscopically intact (MIT) region, while SPP1, KLF4, MAPLC3B, CD68, and LGALS3 were found significantly upregulated in plaque VSMCs versus MIT VSMCs. The gene expression pattern of arterial VSMCs from a healthy donor treated with 7-ketocholesterol showed high similarity with the expression pattern of carotid plaque VSMCs. Our results indicate that VSMCs isolated from plaque show a typical SMC dedifferentiated phenotype with macrophage-like features compared with VSMCs isolated from a MIT region of the carotid artery. Additionally, MYH11, KLF5, and SPP1 expression patterns were found to be associated with symptomatology of human carotid atherosclerosis. | es_ES |
dc.description.sponsorship | This work was supported by Spanish Institute for Health Carlos III, RETICS program (Grant Number RD16/0019/0007) and by Basque Government, Education Department, Consolidated Groups program (Grant Number IT512-10). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | carotid atherosclerosis | es_ES |
dc.subject | plaque instability | es_ES |
dc.subject | smooth muscle cells | es_ES |
dc.subject | MYH11 macrophage-like cells | es_ES |
dc.subject | therapeutic strategies | es_ES |
dc.subject | foam-cell | es_ES |
dc.subject | atherosclerosis | es_ES |
dc.subject | modulation | es_ES |
dc.subject | plaque | es_ES |
dc.subject | contractile | es_ES |
dc.subject | atherogenesis | es_ES |
dc.subject | expression | es_ES |
dc.subject | biology | es_ES |
dc.title | Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.mdpi.com/2073-4409/7/3/23 | es_ES |
dc.identifier.doi | 10.3390/cells7030023 | |
dc.departamentoes | Neurociencias | es_ES |
dc.departamentoeu | Neurozientziak | es_ES |