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A Morphological Method for Ammonia Detection in Liver
dc.contributor.author | Gutiérrez de Juan, Virginia | |
dc.contributor.author | López de Davalillo, Sergio | |
dc.contributor.author | Fernández Ramos, David | |
dc.contributor.author | Barbier Torres, Lucía | |
dc.contributor.author | Zubiete Franco, Imanol | |
dc.contributor.author | Fernández Tussy, Pablo | |
dc.contributor.author | Simón Espinosa, Jorge | |
dc.contributor.author | Lopitz Otsoa, Fernando | |
dc.contributor.author | De las Heras Montero, Javier Adolfo | |
dc.contributor.author | Iruzubieta, Paula | |
dc.contributor.author | Arias Loste, María Teresa | |
dc.contributor.author | Villa, Erica | |
dc.contributor.author | Crespo, Javier | |
dc.contributor.author | Andrade, Raúl | |
dc.contributor.author | Lucena, M. Isabel | |
dc.contributor.author | Varela Rey, Marta | |
dc.contributor.author | Lu, Shelly C. | |
dc.contributor.author | Mato, José M. | |
dc.contributor.author | Cardoso Delgado, Teresa de Jesús | |
dc.contributor.author | Martínez Chantar, María Luz | |
dc.date.accessioned | 2019-05-06T07:38:39Z | |
dc.date.available | 2019-05-06T07:38:39Z | |
dc.date.issued | 2017-03-20 | |
dc.identifier.citation | Plos One 12(3) : (2017) // Article ID e0173914 | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/32644 | |
dc.description.abstract | Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/ failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler A s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome. | es_ES |
dc.description.sponsorship | This work was supported by grants from NIH (USDepartment of Health and Human services)-R01AT001576 (to S.C.L., J.M.M and M.L.M-C.), Gobierno Vasco-Departamento de Salud 2013111114 (to M.L.M.-C), MINECO: SAF20145 4658-R and SAF2014-52097-R integrado en el Plan Estatal de Investigacion Cientifica y Tecnica y Innovacio 2013-2016 cofinanciado con Fondos FEDER to M.L.M.-C and J.M.M. respectively, Instituto de Salud Carlos III:PIE14/00031, integrado en el Plan Estatal de Investigacion Cientifica y Tecnica y Innovacion 2013-2016 cofinanciado con Fondos FEDER (toM.L.M.-C and J.M.M),EITB. BIO15/CA/014, Asociacion Espanola contra el Cancer (T.C.D, P.F-Tand M.L.M-C). Ciberehd_ISCIII_MINECO is funded by the Instituto de Salud Carlos III.Thefunders had no role in study design,datacollection and analysis,decision to publish,or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library Science | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF20145 4658-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2014-52097-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/PIE14/00031 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/ | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | hepatic-encephalopathy | es_ES |
dc.subject | arterial ammonia | es_ES |
dc.subject | cirrhosis | es_ES |
dc.subject | hyperammonemia | es_ES |
dc.subject | failure | es_ES |
dc.subject | complications | es_ES |
dc.subject | hypertension | es_ES |
dc.subject | cells | es_ES |
dc.subject | risk | es_ES |
dc.title | A Morphological Method for Ammonia Detection in Liver | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Attribution 4.0 International (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0173914 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0173914 | |
dc.departamentoes | Pediatría | es_ES |
dc.departamentoeu | Pediatria | es_ES |
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Bestelakorik adierazi ezean, itemaren baimena horrela deskribatzen da:This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Attribution 4.0 International (CC BY 4.0)