Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
dc.contributor.author | Cervantes, Blanca | |
dc.contributor.author | Arana Urbieta, Lide | |
dc.contributor.author | Murillo Cuesta, Silvia | |
dc.contributor.author | Bruno, Marina | |
dc.contributor.author | Alcorta Calvo, Miren Itziar | |
dc.contributor.author | Varela Nieto, Isabel | |
dc.date.accessioned | 2019-12-30T10:15:39Z | |
dc.date.available | 2019-12-30T10:15:39Z | |
dc.date.issued | 2019-09-14 | |
dc.identifier.citation | Journal Of Clinical Medicine 8(9) : (2019) // Article ID 1464 | es_ES |
dc.identifier.issn | 2077-0383 | |
dc.identifier.uri | http://hdl.handle.net/10810/37420 | |
dc.description.abstract | Cisplatin is a chemotherapeutic agent that causes the irreversible death of auditory sensory cells, leading to hearing loss. Local administration of cytoprotective drugs is a potentially better option co-therapy for cisplatin, but there are strong limitations due to the difficulty of accessing the inner ear. The use of nanocarriers for the efficient delivery of drugs to auditory cells is a novel approach for this problem. Solid lipid nanoparticles (SLNs) are biodegradable and biocompatible nanocarriers with low solubility in aqueous media. We show here that stearic acid-based SLNs have the adequate particle size, polydispersity index and zeta-potential, to be considered optimal nanocarriers for drug delivery. Stearic acid-based SLNs were loaded with the fluorescent probe rhodamine to show that they are efficiently incorporated by auditory HEI-OC1 (House Ear Institute-Organ of Corti 1) cells. SLNs were not ototoxic over a wide dose range. Glucocorticoids are used to decrease cisplatin-induced ototoxicity. Therefore, to test SLNs' drug delivery efficiency, dexamethasone and hydrocortisone were tested either alone or loaded into SLNs and tested in a cisplatin-induced ototoxicity in vitro assay. Our results indicate that the encapsulation in SLNs increases the protective effect of low doses of hydrocortisone and lengthens the survival of HEI-OC1 cells treated with cisplatin | es_ES |
dc.description.sponsorship | B.C. was supported by an MSC postdoctoral contract, FP7-PEOPLE2013-IAPP TARGEAR. This work was supported by grants MINECO/FEDER (SAF2017-86107-R) and Comunidad de Madrid/FEDER MULTITARGET& VIEW-CM (B2017/BMD-3688) to I.V.N. and ELKARTEK (2017 KK-2017/00008) to I.A. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/SAF2017-86107-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | nanocarriers | es_ES |
dc.subject | dexamethasone | es_ES |
dc.subject | hydrocortisone | es_ES |
dc.subject | otic protection | es_ES |
dc.subject | stearic acid-based solid lipid nanoparticle (SLN) | es_ES |
dc.subject | sensorineural hearing-loss | es_ES |
dc.subject | intratympanic dexamethasone | es_ES |
dc.subject | drug-delivery | es_ES |
dc.subject | polymeric nanoparticles | es_ES |
dc.subject | cancer-therapy | es_ES |
dc.subject | HEI-OC1 cells | es_ES |
dc.subject | mechanisms | es_ES |
dc.subject | management | es_ES |
dc.subject | sustains | es_ES |
dc.subject | carriers | es_ES |
dc.title | Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Attribution 4.0 International (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.mdpi.com/2077-0383/8/9/1464 | es_ES |
dc.identifier.doi | 10.3390/jcm8091464 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoes | Derecho civil | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.departamentoeu | Zuzenbide zibila | es_ES |
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Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Attribution 4.0 International (CC BY 4.0)