Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
dc.contributor.author | De la Peña Cuao, Marlon | |
dc.contributor.author | González Moro, María Begoña | |
dc.contributor.author | Marino Bilbao, Daniel | |
dc.date.accessioned | 2020-02-10T09:13:36Z | |
dc.date.available | 2020-02-10T09:13:36Z | |
dc.date.issued | 2019-05-10 | |
dc.identifier.citation | AOB Plants 11(3) : (2019) // Article ID plz029 | es_ES |
dc.identifier.issn | 2041-2851 | |
dc.identifier.uri | http://hdl.handle.net/10810/40529 | |
dc.description.abstract | Plants mainly acquire N from the soil in the form of nitrate (NO3-) or ammonium (NH4+). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO3- and indeed, when growing only with NH4+ they can encounter so-called ammonium stress. Since Brachypodium distachyon is a useful model species for the study of monocot physiology and genetics, we chose it to characterize performance under ammonium nutrition. Brachypodium distachyon Bd21 plants were grown hydroponically in 1 or 2.5 mM NO3- or NH4+. Nitrogen and carbon metabolism associated with NH4+ assimilation was evaluated in terms of tissue contents of NO3-, NH4+, K, Mg, Ca, amino acids and organic acids together with tricarboxylic acid (TCA) cycle and NH4+-assimilating enzyme activities and RNA transcript levels. The roots behaved as a physiological barrier preventing NH4+ translocation to aerial parts, as indicated by a sizeable accumulation of NH4+, Asn and Gln in the roots. A continuing high NH4+ assimilation rate was made possible by a tuning of the TCA cycle and its associated anaplerotic pathways to match 2-oxoglutarate and oxaloacetate demand for Gln and Asn synthesis. These results show B. distachyon to be a highly suitable tool for the study of the physiological, molecular and genetic basis of ammonium nutrition in cereals. | es_ES |
dc.description.sponsorship | This research was funded/supported by the Basque Government (IT932-16) and the Spanish Ministry of Economy and Competitiveness (BIO2017-84035-R co-funded by FEDER). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/BIO2017-84035-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | ammonium assimilation | es_ES |
dc.subject | Asn | es_ES |
dc.subject | carbon metabolism | es_ES |
dc.subject | Gln | es_ES |
dc.subject | monocots | es_ES |
dc.subject | nitrate | es_ES |
dc.subject | nitrogen metabolism | es_ES |
dc.subject | root | es_ES |
dc.subject | TCA cycle | es_ES |
dc.subject | glutamine-synthetase | es_ES |
dc.subject | phosphoenolpyruvate carboxylase | es_ES |
dc.subject | arabidopsis-thaliana | es_ES |
dc.subject | gene-expression | es_ES |
dc.subject | plant nitrogen | es_ES |
dc.subject | use efficiency | es_ES |
dc.subject | crucial role | es_ES |
dc.subject | tolerance | es_ES |
dc.subject | toxicity | es_ES |
dc.subject | metabolism | es_ES |
dc.title | Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://academic.oup.com/aobpla/article/11/3/plz029/5487766 | es_ES |
dc.identifier.doi | 10.1093/aobpla/plz029 | |
dc.departamentoes | Biología vegetal y ecología | es_ES |
dc.departamentoeu | Landaren biologia eta ekologia | es_ES |
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