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Magnetosomes could be protective shields against metal stress in magnetotactic bacteria
dc.contributor.author | Muñoz Rodríguez, D. | |
dc.contributor.author | Marcano Prieto, Lourdes | |
dc.contributor.author | Martín Rodríguez, R. | |
dc.contributor.author | Simonelli, L. | |
dc.contributor.author | Serrano, A. | |
dc.contributor.author | García Prieto, Ana | |
dc.contributor.author | Fernández Gubieda Ruiz, María Luisa | |
dc.contributor.author | Muela Blázquez, Alicia | |
dc.date.accessioned | 2020-10-23T12:22:37Z | |
dc.date.available | 2020-10-23T12:22:37Z | |
dc.date.issued | 2020-07 | |
dc.identifier.citation | Scientific Reports 10 : (2020) // Article ID 11430 | es_ES |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10810/47268 | |
dc.description.abstract | Magnetotactic bacteria are aquatic microorganisms with the ability to biomineralise membrane-enclosed magnetic nanoparticles, called magnetosomes. These magnetosomes are arranged into a chain that behaves as a magnetic compass, allowing the bacteria to align in and navigate along the Earth's magnetic field lines. According to the magneto-aerotactic hypothesis, the purpose of producing magnetosomes is to provide the bacteria with a more efficient movement within the stratified water column, in search of the optimal positions that satisfy their nutritional requirements. However, magnetosomes could have other physiological roles, as proposed in this work. Here we analyse the role of magnetosomes in the tolerance of Magnetospirillum gryphiswaldense MSR-1 to transition metals (Co, Mn, Ni, Zn, Cu). By exposing bacterial populations with and without magnetosomes to increasing concentrations of metals in the growth medium, we observe that the tolerance is significantly higher when bacteria have magnetosomes. The resistance mechanisms triggered in magnetosome-bearing bacteria under metal stress have been investigated by means of x-ray absorption near edge spectroscopy (XANES). XANES experiments were performed both on magnetosomes isolated from the bacteria and on the whole bacteria, aimed to assess whether bacteria use magnetosomes as metal storages, or whether they incorporate the excess metal in other cell compartments. Our findings reveal that the tolerance mechanisms are metal-specific: Mn, Zn and Cu are incorporated in both the magnetosomes and other cell compartments; Co is only incorporated in the magnetosomes, and Ni is incorporated in other cell compartments. In the case of Co, Zn and Mn, the metal is integrated in the magnetosome magnetite mineral core. | es_ES |
dc.description.sponsorship | The Spanish and Basque Governments are acknowledged for funding under projects number MAT2017-83631-C3-R and IT-1245-19, respectively. Dr. L. Marcano acknowledges the financial support provided through a postdoctoral fellowship from the Basque Government. The authors acknowledge the technical and human support provided by SGIker (UPV/EHU). The ESRF, MCIU, and CSIC are acknowledged for the provision of ESRF synchrotron radiation facilities. The authors thank Dr. Carlo Meneghini for providing the XANES spectra of Zn standards and R. Fernandez-Pacheco for his assistance in the EDS measurements. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/MAT2017-83631-C3-R | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | manganese ferrite nanoparticles | es_ES |
dc.subject | escherichia-coli | es_ES |
dc.subject | absorption | es_ES |
dc.subject | homeostasis | es_ES |
dc.subject | resistance | es_ES |
dc.subject | growth | es_ES |
dc.subject | xanes | es_ES |
dc.subject | zinc | es_ES |
dc.title | Magnetosomes could be protective shields against metal stress in magnetotactic bacteria | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-020-68183-z#rightslink | es_ES |
dc.identifier.doi | 10.1038/s41598-020-68183-z | |
dc.departamentoes | Electricidad y electrónica | es_ES |
dc.departamentoes | Física aplicada I | es_ES |
dc.departamentoes | Inmunología, microbiología y parasitología | es_ES |
dc.departamentoeu | Elektrizitatea eta elektronika | es_ES |
dc.departamentoeu | Fisika aplikatua I | es_ES |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | es_ES |
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