Multi-Target Analysis and Suspect Screening of Xenobiotics in Milk by UHPLC-HRMS/MS
dc.contributor.author | Musatadi Larrucea, Mikel | |
dc.contributor.author | González Gaya, Belén | |
dc.contributor.author | Irazola Duñabeitia, Mireia | |
dc.contributor.author | Prieto Sobrino, Ailette | |
dc.contributor.author | Etxebarria Loizate, Nestor | |
dc.contributor.author | Olivares Zabalandicoechea, Maitane | |
dc.contributor.author | Zuloaga Zubieta, Olatz | |
dc.date.accessioned | 2021-03-03T11:18:12Z | |
dc.date.available | 2021-03-03T11:18:12Z | |
dc.date.issued | 2021-01-26 | |
dc.identifier.citation | Separations 8(2) : (2021) // Article ID 14 | es_ES |
dc.identifier.issn | 2297-8739 | |
dc.identifier.uri | http://hdl.handle.net/10810/50436 | |
dc.description.abstract | The development of suspect or non-target screening methods to detect xenobiotics in biological fluids is essential to properly understand the exposome and assess its adverse health effects on humans. In order to fulfil that aim, the biomonitorization of human fluids is compulsory. However, these methods are not yet extensively developed, especially for polar organic xenobiotics in biofluids such as milk, as most works are only focused on certain analytes of interest. In this work, a multi-target analysis method to determine 245 diverse xenobiotics in milk by means of Ultra High Performance Liquid Chromatography (UHPLC)-qOrbitrap was developed. Under optimal conditions, liquid milk samples were extracted with acetonitrile in the presence of anhydrous Na2SO4 and NaCl, and the extracts were cleaned-up by protein precipitation at low temperature and Captiva Non-Drip (ND)—Lipids filters. The optimized method was validated at two concentration-levels (10 ng/g and 40 ng/g) obtaining satisfactory figures of merit for more than 200 compounds. The validated multi-target method was applied to several milk samples, including commercial and breast milk, provided by 4 healthy volunteers. Moreover, the method was extended to perform suspect analysis of more than 17,000 xenobiotics. All in all, several diverse xenobiotics were detected, highlighting food additives (benzothiazole) or phytoestrogens (genistein and genistin) in commercial milk samples, and stimulants (caffeine), plasticizers (phthalates), UV filters (benzophenone), or pharmaceuticals (orlistat) in breast milk samples. | es_ES |
dc.description.sponsorship | This research was funded by the Agencia Estatal de Investigación (AEI) of Spain, the European Regional Development Fund through projects CTM2017-84763-C3-1-R and CTM2017-90890-REDT (AEI/FEDER, EU) and the Basque Government through the financial support as consolidated group of the Basque Research System (IT1213-19). B.G. acknowledges a Juan de la Cierva-Formación fellowship by the Spanish Ministry for the Economy, Industry and Competitiveness (MINECO). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CTM2017-84763-C3-1-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/CTM2017-90890-REDT | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | xenobiotics | es_ES |
dc.subject | multi-target analysis | es_ES |
dc.subject | suspect screening | es_ES |
dc.subject | UHPLC-qOrbitrap | es_ES |
dc.subject | commercial milk | es_ES |
dc.subject | breast milk | es_ES |
dc.title | Multi-Target Analysis and Suspect Screening of Xenobiotics in Milk by UHPLC-HRMS/MS | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-02-26T14:43:20Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2297-8739/8/2/14/htm | es_ES |
dc.identifier.doi | 10.3390/separations8020014 | |
dc.departamentoes | Química analítica | |
dc.departamentoeu | Kimika analitikoa |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).