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dc.contributor.authorLadero Auñón, Iraia
dc.contributor.authorMolina, Elena
dc.contributor.authorOyanguren, Maddi
dc.contributor.authorOyanguren Arizaga, Maddi
dc.contributor.authorBarriales, Diego
dc.contributor.authorFuertes, Miguel
dc.contributor.authorSevilla, Iker A.
dc.contributor.authorArrazuria Fernández, Rakel
dc.contributor.authorDe Buck, Jeroen
dc.contributor.authorAnguita Castillo, Juan de Dios
dc.contributor.authorElguezabal Vega, Natalia María
dc.date.accessioned2021-10-25T07:40:52Z
dc.date.available2021-10-25T07:40:52Z
dc.date.issued2021-08-12
dc.identifier.citationNPJ Vaccines 6(1) : (2021) // Article ID 102es_ES
dc.identifier.issn2059-0105
dc.identifier.urihttp://hdl.handle.net/10810/53613
dc.description.abstractAbstract Mycobacterium avium subsp. paratuberculosis (Map) causes paratuberculosis (PTB), a granulomatous enteritis in ruminants that exerts high economic impact on the dairy industry worldwide. Current vaccines have shown to be cost-effective against Map and in some cases confer beneficial non-specific effects against other pathogens suggesting the existence of trained immunity. Although Map infection is mainly transmitted by the fecal-oral route, oral vaccination has not been deeply studied. Therefore, the aim of this study was to compare the oral route with a set of mycobacterial and non-mycobacterial vaccines with a subcutaneously administered commercially available vaccine. Training effects on polymorphonuclear neutrophils (PMNs) and homologous and heterologous in vivo protection against Map were investigated in the rabbit infection model. Oral vaccination with inactivated or live vaccines was able to activate mucosal immunity as seen by elevation of serum IgA and the expression of IL4 in peripheral blood mononuclear cells (PBMCs). In addition, peripheral PMN phagocytosis against Map was enhanced by vaccination and extracellular trap release against Map and non-related pathogens was modified by both, vaccination and Map-challenge, indicating trained immunity. Finally, PBMCs from vaccinated animals stimulated in vitro with Map antigens showed a rapid innate activation cytokine profile. In conclusion, our data show that oral vaccination against PTB can stimulate neutrophil activity and both innate and adaptive immune responses that correlate with protection.es_ES
dc.description.sponsorshipThe research was funded by the Department of Economy, Sustainability and Environment of the Basque Government and by grant RTA 2017-00089-00-00 of the National Institute for Agronomic Research (INIA) to N.E. I.L.-A. and M.O. both held predoctoral fellowships from the DEI of the Basque Government. This research was also partly supported by the Agriculture Funding Consortium members Alberta Agriculture and Forestry and Alberta Milk (2018F019R) to J.D.B. CIC bioGUNE thanks the Ministry of Science and Innovation for the Severo Ochoa excellence award (SEV-2016-0644)es_ES
dc.language.isoenges_ES
dc.publisherNaturees_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/SEV-2016-0644es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectextracellular trapses_ES
dc.subjectantibody-responsees_ES
dc.subjectcattlees_ES
dc.subjectPCRes_ES
dc.subjectactivationes_ES
dc.subjectinductiones_ES
dc.subjectIL-10es_ES
dc.subjectbovises_ES
dc.subjectLPSes_ES
dc.titleOral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection modeles_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.holderThis article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)es_ES
dc.rights.holderAtribución 3.0 España*
dc.relation.publisherversionhttps://www-nature-com.ehu.idm.oclc.org/articles/s41541-021-00367-8es_ES
dc.identifier.doi10.1038/s41541-021-00367-8
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES


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This article is licensed under a Creative Commons Attribution 4.0 International License  (CC BY 4.0)
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