Aspergillus fumigatus Fumagillin Contributes to Host Cell Damage
dc.contributor.author | Guruceaga Sierra, Xabier | |
dc.contributor.author | Pérez Cuesta, Uxue | |
dc.contributor.author | Pellón Rodríguez, Aize | |
dc.contributor.author | Cendón Sánchez, Saioa | |
dc.contributor.author | Pelegrí Martínez, Eduardo | |
dc.contributor.author | González Mendia, Oscar | |
dc.contributor.author | Hernando Echevarria, Fernando Luis | |
dc.contributor.author | Mayayo, Emilio | |
dc.contributor.author | Anguita Castillo, Juan de Dios | |
dc.contributor.author | Alonso Rojas, Rosa María | |
dc.contributor.author | Keller, Nancy P. | |
dc.contributor.author | Ramírez García, Andoni | |
dc.contributor.author | Rementeria Ruiz, Aitor Domingo | |
dc.date.accessioned | 2021-11-29T12:39:31Z | |
dc.date.available | 2021-11-29T12:39:31Z | |
dc.date.issued | 2021-11-03 | |
dc.identifier.citation | Journal of Fungi 7(11) : (2021) // Article ID 936 | es_ES |
dc.identifier.issn | 2309-608X | |
dc.identifier.uri | http://hdl.handle.net/10810/54176 | |
dc.description.abstract | The activity of fumagillin, a mycotoxin produced by Aspergillus fumigatus, has not been studied in depth. In this study, we used a commercial fumagillin on cultures of two cell types (A549 pneumocytes and RAW 264.7 macrophages). This toxin joins its target, MetAP2 protein, inside cells and, as a result, significantly reduces the electron chain activity, the migration, and the proliferation ability on the A549 cells, or affects the viability and proliferation ability of the RAW 264.7 macrophages. However, the toxin stimulates the germination and double branch hypha production of fungal cultures, pointing out an intrinsic resistant mechanism to fumagillin of fungal strains. In this study, we also used a fumagillin non-producer A. fumigatus strain (∆fmaA) as well as its complemented strain (∆fmaA::fmaA) and we tested the fumagillin secretion of the fungal strains using an Ultra High-Performance Liquid Chromatography (UHPLC) method. Furthermore, fumagillin seems to protect the fungus against phagocytosis in vitro, and during in vivo studies using infection of immunosuppressed mice, a lower fungal burden in the lungs of mice infected with the ∆fmaA mutant was demonstrated. | es_ES |
dc.description.sponsorship | This research was funded by the Basque Government: grant number IT1362-19. X.G. and S.C.-S. received a Ph.D. fellowship from the Basque Government; and U.P.-C. from the University of the Basque Country. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject | fumagillin | es_ES |
dc.subject | Aspergillus fumigatus | es_ES |
dc.subject | virulence factor | es_ES |
dc.subject | pathogenesis | es_ES |
dc.subject | RAW 264.7 | es_ES |
dc.subject | A549 | es_ES |
dc.subject | mice infection | es_ES |
dc.subject | UHPLC | es_ES |
dc.title | Aspergillus fumigatus Fumagillin Contributes to Host Cell Damage | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2021-11-25T15:59:58Z | |
dc.rights.holder | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/2309-608X/7/11/936/htm | es_ES |
dc.identifier.doi | 10.3390/jof7110936 | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoes | Química analítica | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | |
dc.departamentoeu | Kimika analitikoa |
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Except where otherwise noted, this item's license is described as 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).