Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing

Las Heras Zapata, Kevin; Royo, Félix; García Vallicrosa, Clara; Igartua Olaechea, Manuela; Santos Vizcaíno, Edorta; Falcón Pérez, Juan Manuel; Hernández Martín, Rosa María

dc.contributor.author	Las Heras Zapata, Kevin
dc.contributor.author	Royo, Félix
dc.contributor.author	García Vallicrosa, Clara
dc.contributor.author	Igartua Olaechea, Manuela
dc.contributor.author	Santos Vizcaíno, Edorta
dc.contributor.author	Falcón Pérez, Juan Manuel
dc.contributor.author	Hernández Martín, Rosa María
dc.date.accessioned	2022-05-02T07:51:10Z
dc.date.available	2022-05-02T07:51:10Z
dc.date.issued	2022-04
dc.identifier.citation	Stem Cell Research & Therapy 13 : (2022) // Article ID 147	es_ES
dc.identifier.issn	1757-6512
dc.identifier.uri	http://hdl.handle.net/10810/56452
dc.description.abstract	[EN] Background Mesenchymal stromal cells (MSCs) and their extracellular vesicles (MSC-EVs) have demonstrated to elicit immunomodulatory and pro-regenerative properties that are beneficial for the treatment of chronic wounds. Thanks to different mediators, MSC-EVs have shown to play an important role in the proliferation, migration and cell survival of different skin cell populations. However, there is still a big bid to achieve the most effective, suitable and available source of MSC-EVs. Methods We isolated, characterized and compared medium-large EVs (m-lEVs) and small EVs (sEVs) obtained from hair follicle-derived MSCs (HF-MSCs) against the gold standard in regenerative medicine, EVs isolated from adipose tissue-derived MSCs (AT-MSCs). Results We demonstrated that HF-EVs, as well as AT-EVs, expressed typical MSC-EVs markers (CD9, CD44, CD63, CD81 and CD105) among other different functional markers. We showed that both cell types were able to increase human dermal fibroblasts (HDFs) proliferation and migration. Moreover, both MSC-EVs were able to increase angiogenesis in human umbilical vein endothelial cells (HUVECs) and protect HDFs exposed to a hyperglycemic environment from oxidative stress and cytotoxicity. Conclusions Taken together, HF-EVs demonstrated to exhibit comparable potential to that of AT-EVs as promising candidates in the treatment of chronic wounds.	es_ES
dc.description.sponsorship	This research was funded by the Basque Government (Consolidated Groups, IT907-16 and PRE_2018_1_0412).	es_ES
dc.language.iso	eng	es_ES
dc.publisher	BMC	es_ES
dc.rights	info:eu-repo/semantics/openAccess	es_ES
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/es/	*
dc.subject	extracellular vesicles	es_ES
dc.subject	exosomes	es_ES
dc.subject	microvesicles	es_ES
dc.subject	mesenchymal stromal cells	es_ES
dc.subject	chronic wounds	es_ES
dc.subject	skin wounds	es_ES
dc.subject	hair follicle	es_ES
dc.subject	diabetic ulcers	es_ES
dc.title	Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.rights.holder	© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.	es_ES
dc.rights.holder	Atribución 3.0 España	*
dc.relation.publisherversion	https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-02824-0	es_ES
dc.identifier.doi	10.1186/s13287-022-02824-0
dc.departamentoes	Farmacia y ciencias de los alimentos	es_ES
dc.departamentoeu	Farmazia eta elikagaien zientziak	es_ES

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© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which
permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the
original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or
other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line
to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this
licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco
mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Except where otherwise noted, this item's license is described as © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.