dc.contributor.author | Pérez García, Javier | |
dc.contributor.author | González Carracedo, Mario | |
dc.contributor.author | Espuela Ortiz, Antonio | |
dc.contributor.author | Hernández Pérez, José M. | |
dc.contributor.author | González Pérez, Ruperto | |
dc.contributor.author | Sardón Prado, Olaia | |
dc.contributor.author | Martín González, Elena | |
dc.contributor.author | Mederos Luis, Elena | |
dc.contributor.author | Poza Guedes, Paloma | |
dc.contributor.author | Corcuera Elosegui, Paula | |
dc.contributor.author | Callero, Ariel | |
dc.contributor.author | Sánchez Machín, Inmaculada | |
dc.contributor.author | Korta Murua, José Javier | |
dc.contributor.author | Pérez Pérez, José Antonio | |
dc.contributor.author | Villar, Jesús | |
dc.contributor.author | Pino Yanes, María | |
dc.contributor.author | Lorenzo Díaz, Fabián | |
dc.date.accessioned | 2023-03-21T16:36:31Z | |
dc.date.available | 2023-03-21T16:36:31Z | |
dc.date.issued | 2023-03 | |
dc.identifier.citation | Journal of Allergy and Clinical Immunology 151(3) : 706-715 (2023) | es_ES |
dc.identifier.issn | 1097-6825 | |
dc.identifier.uri | http://hdl.handle.net/10810/60433 | |
dc.description.abstract | BACKGROUND: The response to inhaled corticosteroids (ICS) in asthma is affected by the interplay of several factors. Among these, the role of the upper-airway microbiome has been scarcely investigated. We aimed to evaluate the association between the salivary, pharyngeal, and nasal microbiome with asthma exacerbations despite receipt of ICS.
METHODS: Samples from 250 asthma patients from the Genomics and Metagenomics of Asthma Severity (GEMAS) study treated with ICS were analyzed. Control/case subjects were defined by the absence/presence of asthma exacerbations in the past 6 months despite being treated with ICS. The bacterial microbiota was profiled by sequencing the V3-V4 region of the 16S rRNA gene. Differences between groups were assessed by PERMANOVA and regression models adjusted for potential confounders. Afalse discovery rate (FDR) of 5% was used to correct for multiple comparisons. Classification models of asthma exacerbations despite ICS treatment were built with machine learning approaches based on clinical, genetic, and microbiome data.
RESULTS: In nasal and saliva samples, case subjects had lower bacterial diversity (Richness, Shannon, and Faith indices) than control subjects (.007≤ P≤ .037). Asthma exacerbations accounted for 8% to 9% of the interindividual variation of the salivary and nasal microbiomes (.003≤ P≤ .046). Three, 4, and 11 bacterial genera from the salivary, pharyngeal, and nasal microbiomes were differentially abundant between groups (4.09*10-12≤ FDR≤ 0.047). Integrating clinical, genetic, and microbiome data showed good discrimination for the development of asthma exacerbations despite receipt of ICS (AUCtraining: 0.82 and AUCvalidation: 0.77).
CONCLUSION: The diversity and composition of the upper-airway microbiome are associated with asthma exacerbations despite ICS treatment. The salivary microbiome has a potential application as a biomarker of asthma exacerbations despite receipt of ICS. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | 16S rRNA | es_ES |
dc.subject | asthma | es_ES |
dc.subject | biomarker | es_ES |
dc.subject | exacerbations | es_ES |
dc.subject | inhaled corticosteroids | es_ES |
dc.subject | microbiota | es_ES |
dc.subject | nasal | es_ES |
dc.subject | pharyngeal | es_ES |
dc.subject | precision medicine | es_ES |
dc.subject | saliva | es_ES |
dc.title | The upper-airway microbiome as a biomarker of asthma exacerbations despite inhaled corticosteroid treatment. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | © 2022 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy,
Asthma & Immunology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0091674922014750?via%3Dihub | es_ES |
dc.identifier.doi | 10.1016/j.jaci.2022.09.041 | |
dc.departamentoes | Pediatría | es_ES |
dc.departamentoeu | Pediatria | es_ES |