Co-Existence of blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt
dc.contributor.author | Sánchez Urtaza, Sandra | |
dc.contributor.author | Ocampo Sosa, Alain | |
dc.contributor.author | Molins Bengoetxea, Ainhoa | |
dc.contributor.author | Rodríguez Grande, Jorge | |
dc.contributor.author | El-Kholy, Mohammed A. | |
dc.contributor.author | Hernández, Marta | |
dc.contributor.author | Abad, David | |
dc.contributor.author | Shawky, Sherine M. | |
dc.contributor.author | Alcorta Calvo, Miren Itziar | |
dc.contributor.author | Gallego Andrés, Lucía | |
dc.date.accessioned | 2023-08-28T07:50:36Z | |
dc.date.available | 2023-08-28T07:50:36Z | |
dc.date.issued | 2023-08-07 | |
dc.identifier.citation | International Journal of Molecular Sciences 24(15) : (2023) // Article ID 12515 | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/10810/62236 | |
dc.description.abstract | The increasing rates of antimicrobial resistance among carbapenem-resistant Acinetobacter baumannii in the Middle East and North Africa are one of the major concerns for healthcare settings. We characterised the first A. baumannii isolate harbouring five β-lactamases identified in Egypt. The isolate Ale25 was obtained from an ICU patient of a hospital from Alexandria. The isolate was phenotypically and genotypically screened for carbapenemase genes. The isolate was resistant to carbapenems, aminoglycosides, fluoroquinolones and cefiderocol. Whole-Genome Sequencing identified five β-lactamase genes, blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57, together with other antibiotic resistance genes, conferring resistance to sulfonamides, macrolides, tetracyclines, rifamycin and chloramphenicol. Virulome analysis showed the presence of genes involved in adhesion and biofilm production, type II and VI secretion systems, exotoxins, etc. Multi-Locus Sequence Typing analysis identified the isolate as Sequence Types 113Pas and 2246Oxf, belonging to International Clone 7. Sequencing experiments revealed the presence of four plasmids of 2.7, 22.3, 70.4 and 240.8 Kb. All the β-lactamase genes were located in the chromosome, except the blaPER-7, gene which was found within the plasmid of 240.8 Kb. This study highlights the threat of the emergence and dissemination of these types of isolates. | es_ES |
dc.description.sponsorship | This research was funded by the MINISTRY OF SCIENCE AND INNOVATION (MCIN/AEI/10.13039/501100011033), grant number PID2020-116495RB-I00; the DEPARTMENT OF EDUCATION OF THE BASQUE GOVERNMENT (Research Groups of the Basque University System 2021), grant number Group IT1578-22, GIC21/18; and the ARAB ACADEMY FOR SCIENCE, TECHNOLOGY AND MARITIME TRANSPORT, grant number 2072. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/PID2020-116495RB-I00 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Acinetobacter baumannii | es_ES |
dc.subject | carbapenemase genes | es_ES |
dc.subject | whole genome sequencing | es_ES |
dc.title | Co-Existence of blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.date.updated | 2023-08-11T14:33:46Z | |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/24/15/12515 | es_ES |
dc.identifier.doi | 10.3390/ijms241512515 | |
dc.departamentoes | Inmunología, microbiología y parasitología | |
dc.departamentoes | Bioquímica y biología molecular | |
dc.departamentoeu | Immunologia, mikrobiologia eta parasitologia | |
dc.departamentoeu | Biokimika eta biologia molekularra |
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Except where otherwise noted, this item's license is described as © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).