Browsing by Author "Barrondo, Sergio"
Now showing items 1-15 of 15
-
2-Arakidonilglizerol endokannabinoidearen sintesi- eta degradazio-makineria neuronaren nukleoan: Diazilglizerol Lipasa alfa-ren konpartimentazioa eta aktibitate entzimatikoa
Arechabala Rodriguez, Xabier (2017-09-29)Ikerketa lan honetan, zatikapen azpizelularra, Western blot eta fluoreszentzia mikroskopia bidez, diazilglizerol lipasa ¿-ren (DAGL¿) presentzia frogatu dugu arratoi helduaren garun-kortexetik isolatutako nukleoetan. ... -
Biochemical and functional charactrization of the CB1 cannabinoid recptor coupling to Gai/l proteins in synaptosomal membranes from hippocampus and frontal cortex of cell-type-specifc mutant mouse rescue model
Saumell Esnaola, Miquel (2021-05-18)Egun, ezaguna da terminal sinaptiko glutamatergiko eta GABAergikoetan espresatzen den CB1 hartzailearen aktibazioak aktibitate neuronal eszitatzaile-inhibitzailearen balantzea erregulatzen duela. Horregatik, bi terminal ... -
Cellular neurochemical characterization and subcellular localization of Phospholipase C-β1 in rat brain
Montaña, Mario; García del Caño, Gontzal; López de Jesús, Maider; González Burguera, Imanol; Echeazarra, Leyre; Barrondo Lacarra, Sergio
; Sallés Alvira, Joan (Elsevier, 2012-10-11)
The present study describes a complete and detailed neuroanatomical distribution map of the phospholipase C beta1 (PLCβ1) isoform along the adult rat neuraxis, and defines the phenotype of cells expressing PLCβ1, along ... -
Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods
Saumell Esnaola, Miquel; Elejaga Jimeno, Ainhoa; Echeazarra Escudero, Leire; Borrega Román, Leire; Barrondo Lacarra, Sergio; López de Jesús, Maider
; González Burguera, Imanol; Gomez-Caballero, Alberto; Goicolea Altuna, María Aranzazu
; Sallés Alvira, Joan; García del Caño, Gontzal (BMC, 2022)
Background: Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis of G protein-coupled receptor (GPCR) levels requires the use of purified protein standards ... -
Determining the binding kinetics and signalling effects of CB1 and CB2 receptor ligands; a closer look at residence time and functional selectivity
Borrega Román, Leire (2022-05-30)The CB1 and CB2 receptors are the main GPCRs of the endocannabinoid system, and regulate many important physiological processed as pain, inflammation, or behaviour. Increasing interest exist to develop new drugs targeting ... -
Endocannabinoid 2-Arachidonoylglycerol Synthesis and Metabolism at Neuronal Nuclear Matrix Fractions Derived from Adult Rat Brain Cortex
Aretxabala Rodríguez, Xabier; García del Caño, Gontzal; Barrondo Lacarra, Sergio; López de Jesús, Maider
; González Burguera, Imanol; Saumell Esnaola, Miquel; Goicolea Altuna, María Aranzazu
; Sallés Alvira, Joan (MDPI, 2023-02-05)
In this report, we describe the kinetics characteristics of the diacylglycerol lipase-α (DGLα) located at the nuclear matrix of nuclei derived from adult cortical neurons. Thus, using high-resolution fluorescence microscopy, ... -
Estudio de utilización de benzodiacepinas y análogos en el tratamiento del insomnio en una Unidad de Ortogeriatría hospitalaria.
Sánchez Monasterio, Iñigo (2021-12-02)[ES] Objetivo: Los ancianos con una fractura de cadera son especialmente vulnerables presentando un aumento del riesgo de caídas, que asociado a benzodiacepinas podría ser aún mayor. El objetivo es analizar la prevalencia ... -
Fit-for-purpose based testing and validation of antibodies to amino- and carboxy-terminal domains of cannabinoid receptor 1
Echeazarra Escudero, Leire; García del Caño, Gontzal; Barrondo Lacarra, Sergio; González Burguera, Imanol; Saumell Esnaola, Miquel; Aretxabala Rodríguez, Xabier; López de Jesús, Maider
; Borrega Román, Leire; Mato Santos, Susana
; Ledent, Catherine; Matute Almau, Carlos José; Goicolea Altuna, María Aranzazu
; Sallés Alvira, Joan (Springer, 2021-11)
Specific and selective anti-CB1 antibodies are among the most powerful research tools to unravel the complex biological processes mediated by the CB1 receptor in both physiological and pathological conditions. However, low ... -
Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization
Saumell Esnaola, Miquel; Delgado San Vicente, Diego; García del Caño, Gontzal; Beitia San Vicente, Maider; Sallés Alvira, Joan; González Burguera, Imanol; Sánchez, Pello; López de Jesús, Maider; Barrondo Lacarra, Sergio
; Sánchez, Mikel (MDPI, 2022-03-05)
Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either in free form or ... -
Ketaminaren eragin antidepresiboak. Ekintzamekanismo berriak farmako antidepresiboen bilaketarako
Borrega Román, Leire; Saumell Esnaola, Miquel; Isasti Iribar, Amaia; Aretxabala, Xabier; González Burguera, Imanol; López de Jesús, Maider; García del Caño, Gontzal; Sallés Alvira, Joan; Barrondo Lacarra, Sergio
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2019)
Beste Medikuntza-arloetan ez bezala, Psikiatrian gaixotasun psikiatrikoei buruzko hipotesi etiologikoak ebi-dentzia farmakologikoetan oinarritzen dira. Horrela, 1950eko hamarkadan depresioaren kontrako farmakoak garatzen ... -
Lack of the transient receptor potential vanilloid 1 shifts cannabinoid-dependent excitatory synaptic plasticity in the dentate gyrus of the mouse brain hippocampus
Egaña Huguet, Jon; Saumell Esnaola, Miquel; Achicallende Urcaregui, Svein; Soria Gómez, Edgar
; Bonilla del Río, Itziar; García del Caño, Gontzal; Barrondo Lacarra, Sergio
; Sallés Alvira, Joan; Gerrikagoitia Marina, Inmaculada
; Puente Bustinza, Nagore
; Elezgarai Gabantxo, Izaskun
; Grandes Moreno, Pedro Rolando
(Frontiers Media, 2021-07-07)
[EN] The transient receptor potential vanilloid 1 (TRPV1) participates in synaptic functions in the brain. In the dentate gyrus, post-synaptic TRPV1 in the granule cell (GC) dendritic spines mediates a type of long-term ... -
Sistema endokannabinoidea: etorkizun handiko itu terapeutikoa gaixotasun psikiatrikoetan
Barrondo Lacarra, Sergio; López de Jesús, Maider
; García del Caño, Gontzal; González Burguera, Imanol; Aretxabala, Xabier; Montaña, Mario; Sallés Alvira, Joan (Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2013)
Sistema endokannabinoideak banaketa zabala dauka giza garunean, eta sistema neuromodulatzailerik garrantzitsuenetako bat izan litekeela proposatu da. Epidemiologi ikasketetan zein animali ereduetan, sistema endokannabinoidearen ... -
Subsynaptic Distribution, Lipid Raft Targeting and G Protein-Dependent Signalling of the Type 1 Cannabinoid Receptor in Synaptosomes from the Mouse Hippocampus and Frontal Cortex
Saumell Esnaola, Miquel; Barrondo Lacarra, Sergio; García del Caño, Gontzal; Goicolea Altuna, María Aranzazu
; Sallés Alvira, Joan; Lutz, Beat; Monory, Krisztina (MDPI, 2021-11-16)
Numerous studies have investigated the roles of the type 1 cannabinoid receptor (CB1) in glutamatergic and GABAergic neurons. Here, we used the cell-type-specific CB1 rescue model in mice to gain insight into the organizational ... -
Teratokartzinometatik eratorritako zelula-lerro neuronalen (NT2N) trasplantea: istripu zerebrobaskularra izandako gaixo kronikoentzako estrategia terapeutiko berria
Isasti Iribar, Amaia; Borrega Román, Leire; Saumell Esnaola, Miquel; González Burguera, Imanol; Barrondo Lacarra, Sergio; Sallés Alvira, Joan; García del Caño, Gontzal; López de Jesús, Maider
(Servicio Editorial de la Universidad del País Vasco/Euskal Herriko Unibertsitatearen Argitalpen Zerbitzua, 2020)
Currently, Cerebrovascular accident (CVA) also known as stroke, is the third leading cause of disability and death worldwide. CVA is a sudden alteration of cerebral circulation, which results in neural cell death and ... -
Up-regulation of CB1 Cannabinoid Receptors Located at Glutamatergic Terminals in the Medial Prefrontal Cortex of the Obese Zucker Rat.
Echeazarra, Leyre; Barrondo Lacarra, Sergio; García del Caño, Gontzal; Bonilla del Río, Itziar; Egaña Huguet, Jon; Puente Bustinza, Nagore
; Aretxabala Rodríguez, Xabier; Montaña, Mario; López de Jesús, Maider
; González Burguera, Imanol; Saumell Esnaola, Miquel; Goicolea Altuna, María Aranzazu
; Grandes Moreno, Pedro Rolando
; Sallés Alvira, Joan (Frontiers Media, 2022-10-18)
The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB1) receptor, along with the biochemical characterization of the expression and functional coupling to their cognate G i/o ...