BackBrowsing by Author "Rujas Díez, Edurne"
Now showing items 1-17 of 17
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Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments
; ; ; ; ; ; Sánchez Eugenia, Rubén
; ; ; ; ; ; ; ; ; ; ; ; (Cell Press, 2020-08-18)
The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ... -
Cholesterol Constrains the Antigenic Configuration of the Membrane-Proximal Neutralizing HIV-1 Epitope
Torralba Iturbe, Johana; De la Arada Echevarría, Igor ; ; ; ; ; ; ; Apellaniz Unzalu, Beatriz ; (ACS, 2020-06-25)
The envelope glycoprotein (Env) enables HIV-1 cell entry through fusion of host-cell and viral membranes induced by the transmembrane subunit gp41. Antibodies targeting the C-terminal sequence of the membrane-proximal ... -
Cholesterol-dependent membrane fusion induced by the gp41 membrane-proximal external region-transmembrane domain connection suggests a mechanism for broad HIV-1 neutralization
Apellaniz Unzalu, Beatriz; ; ; ; ; ; (ASM, 2014)
The HIV-1 glycoprotein 41 promotes fusion of the viral membrane with that of the target cell. Structural, biochemical, and biophysical studies suggest that its membrane-proximal external region (MPER) may interact with the ... -
Dual Inhibition of Vacuolar-ATPase and TMPRSS2 Is Required for Complete Blockade of SARS-CoV-2 Entry into Cells
(American Society for Microbiology, 2022-07)An essential step in the infection life cycle of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the proteolytic activation of the viral spike (S) protein, which enables membrane fusion and entry into ... -
Engineering pan–HIV-1 neutralization potency through multispecific antibody avidity
(National Academy of Sciences of the United States of America, 2022-01-25)Deep mining of B cell repertoires of HIV-1-infected individuals has resulted in the isolation of dozens of HIV-1 broadly neutralizing antibodies (bNAbs). Yet, it remains uncertain whether any such bNAbs alone are sufficiently ... -
Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile
; ; ; ; ; ; ; Sánchez Eugenia, Rubén; ; ; ; ; ; ; ; ; (Cell Press, 2021-09-24)
Broadly neutralizing antibodies (bnAbs) against HIV-1 are frequently associated with the presence of autoreactivity/polyreactivity, a property that can limit their use as therapeutic agents. The bnAb 4E10, targeting the ... -
Functional Delineation of a Protein–Membrane Interaction Hotspot Site on the HIV-1 Neutralizing Antibody 10E8
; ; Torralba Iturbe, Johana; ; ; De la Arada Echevarría, Igor ; Apellaniz Unzalu, Beatriz ; ; ; ; ; (MDPI, 2022-09-15)
Antibody engagement with the membrane-proximal external region (MPER) of the envelope glycoprotein (Env) of HIV-1 constitutes a distinctive molecular recognition phenomenon, the full appreciation of which is crucial for ... -
Mechanisms of MPER helix binding at membrane interfaces and implications for the broad neutralization of HIV by antibodies
(2016-12-16)En esta tesis se lleva a cabo un estudio detallado de la función que desempeña el lazo CDR-H3 de losanticuerpos anti MPER 4E10 y 10E8 en al unión y neutralización del VIH así como la contribución de launión directa a lípidos ... -
Modular adjuvant-free pan-HLA-DR-immunotargeting subunit vaccine against SARS-CoV-2 elicits broad sarbecovirus-neutralizing antibody responses
(Elsevier, 2023-04)Subunit vaccines typically require co-administration with an adjuvant to elicit protective immunity, adding development hurdles that can impede rapid pandemic responses. To circumvent the need for adjuvant in a severe acute ... -
Molecular recognition of a membrane-anchored HIV-1 pan-neutralizing epitope.
Torralba Iturbe, Johana; De la Arada Echevarría, Igor ; ; ; ; ; ; ; ; ; ; Apellaniz Unzalu, Beatriz ; ; (Nature, 2022)
Antibodies against the carboxy-terminal section of the membrane-proximal external region (C-MPER) of the HIV-1 envelope glycoprotein (Env) are considered as nearly pan-neutralizing. Development of vaccines capable of ... -
Molecular Recognition of the Native HIV-1 MPER Revealed by STED Microscopy of Single Virions
; Chojnacki, Jakub; Rujas Díez, Edurne; ; ; Waithe, Dominic; Apellaniz Unzalu, Beatriz; Sicard, Taylor; Julien, Jean Philippe; Eggeling, Christian; (Springer, 2019-01-08)
Antibodies against the Membrane-Proximal External Region (MPER) of the Env gp41 subunit neutralize HIV-1 with exceptional breadth and potency. Due to the lack of knowledge on the MPER native structure and accessibility, ... -
Multivalency Transforms SARS-CoV-2 Antibodies Into Ultrapotent Neutralizers
(Nature, 2021-06-16)SARS-CoV-2, the virus responsible for COVID-19, has caused a global pandemic. Antibodies can be powerful biotherapeutics to fight viral infections. Here, we use the human apoferritin protomer as a modular subunit to drive ... -
On the antimicrobial properties and endurance of eugenol and 2-phenylphenol functionalized sol-gel coatings
(Elsevier, 2024-04)Preventing microbiological surface contamination in public spaces is nowadays of high priority. The proliferation of a microbial infection may arise through air, water, or direct contact with infected surfaces. Chemical ... -
Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface
; ; ; ; ; Apellaniz Unzalu, Beatriz; ; ; ; ; ; (Springer Nature, 2016-12-01)
The mechanism by which the HIV-1 MPER epitope is recognized by the potent neutralizing antibody 10E8 at membrane interfaces remains poorly understood. To solve this problem, we have optimized a 10E8 peptide epitope and ... -
Structural Characterization of the ICOS/ICOS-L Immune Complex Reveals High Molecular Mimicry by Therapeutic Antibodies
(Nature, 2020-10-08)The inducible co-stimulator (ICOS) is a member of the CD28/B7 superfamily, and delivers a positive co-stimulatory signal to activated T cells upon binding to its ligand (ICOS-L). Dysregulation of this pathway has been ... -
Structure and immunogenicity of a peptide vaccine, including the complete HIV-1 gp41 2F5 epitope: implications for antibody recognition mechanism and immunogen design
; ; Apellaniz Unzalu, Beatriz; ; ; De la Arada Echevarría, Igor ; ; ; ; ; ; ; (Elsevier, 2014-01-15)
The membrane-proximal external region (MPER) of gp41 harbors the epitope recognized by the broadly neutralizing anti-HIV 2F5 antibody, a research focus in HIV-1 vaccine development. In this work, we analyze the structure ... -
The Atomic Structure of the HIV-1 gp41 Transmembrane Domain and Its Connection to the Immunogenic Membrane-proximal External Region
Apellaniz Unzalu, Beatriz; ; ; ; ; ; ; (Elsevier, 2015-03-18)
The membrane-proximal external region (MPER) C-terminal segment and the transmembrane domain (TMD) of gp41 are involved in HIV-1 envelope glycoprotein-mediated fusion and modulation of immune responses during viral infection. ...