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dc.contributor.advisorAlcorta Calvo, Miren Itziar
dc.contributor.authorUgarte Uribe, Begoña
dc.contributor.otherBioquímica y Biología Molecular/Biokimika eta Biologia Molekularraes
dc.description399 p. : il.,
dc.description.abstractSmall nucleic acids present a great potential to silence specific genes or inhibit the biological activity of specific proteins. Unfortunately, their poor cellular accessibility presents a big challenge for developing an efficient delivery system. In this regard, conjugation of appropriate molecules to small nucleic improves their pharmacokinetic behaviours and cellular uptake efficiencies, endowing them with entirely new properties. In the present work different carbohydrate- and lipid-oligonucleotide conjugates have been studied as novel tools for targeted delivery and enhancement of cellular permeability. The results obtained in this work indicate that keeping a certain distance between DNA and sugar modification could be important for a better incorporation of this type of conjugates into the target cell. Also long or double-tailed lipid modifications are preferred for an enhanced incorporation of lipid-oligonucleotide conjugates into membrane-model and cell
dc.description.sponsorshipThe present thesis has been performed Departamento de Bioquímica y Biología Molecular de la Universidad del País Vasco and Unidad de Biofísica (Centro Mixto CSIC-UPV/EHU). This work has been supported mainly by CSIC (CARBINH, PIF06-045). The author received a research fellowship from the University of the Basque Country (PIFA01/2006/052, June 2007-May 2011) and a research contract supported by Fundación Biofísica Bizkaia (June 2011-May 2012).es
dc.subjectlipid conjugationes
dc.subjectcarbohydrate modificationes
dc.subjectmembrane modeles
dc.subjectcellular uptakees
dc.subjectdelivery systemes
dc.titleDelivery of small nucleic acids by conjugation to carbohydrates and lipids as novel research and therapeutic toolses
dc.rights.holderAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.departamentoesBioquímica y biología moleculares_ES
dc.departamentoeuBiokimika eta biologia molekularraes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International