VolverExaminar OpenAire por departamento (eus.) "Biokimika eta biologia molekularra"
Mostrando ítems 1-20 de 22
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A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis
Barth, Nicole D.; Subirós Funosas, Ramón; Mendive Tapia, Lorena; Duffin, Rodger; Shields, Mario A.; Cartwright, Jennifer A.; Troeira Henriques, Sónia; Sot, Jesús; Goñi Urcelay, Félix María
; Lavilla, Rodolfo; Marwick, John A.; Vermeren, Sonja; Rossi, Adriano G.; Egeblad, Mikala; Dransfield, Ian; Vendrell, Marc (Nature, 2020-08-12)
Programmed cell death or apoptosis is a central biological process that is dysregulated in many diseases, including inflammatory conditions and cancer. The detection and quantification of apoptotic cells in vivo is hampered ... -
Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments
; ; ; ; ; ; Sánchez Eugenia, Rubén; ; ; ; ; ; ; ; ; ; ; ; (Cell Press, 2020-08-18)
The contribution of membrane interfacial interactions to recognition of membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization of this type of antibodies via chemical ... -
Analysis on the Characterization of Multiphoton Microscopy Images for Malignant Neoplastic Colon Lesion Detection under Deep Learning Methods
Terradillos Fernández, Elena; López Saratxaga, Cristina; Mattana, Sara; Cicchi, Riccardo; Pavone, Francesco S.; Andraka Rueda, Nagore; Glover, Benjamin J.; Arbide del Río, Nagore; Velasco Arteche, Jacques; Etxezarraga Zuluaga, María Carmen; Picón Ruiz, Artzai (Wolters Kluwer, 2021-06-21)
Background: Colorectal cancer has a high incidence rate worldwide, with over 1.8 million new cases and 880,792 deaths in 2018. Fortunately, its early detection significantly increases the survival rate, reaching a cure ... -
Cholesterol Constrains the Antigenic Configuration of the Membrane-Proximal Neutralizing HIV-1 Epitope
Torralba Iturbe, Johana; De la Arada Echevarría, Igor ; ; ; ; ; ; ; Apellaniz Unzalu, Beatriz ; (ACS, 2020-06-25)
The envelope glycoprotein (Env) enables HIV-1 cell entry through fusion of host-cell and viral membranes induced by the transmembrane subunit gp41. Antibodies targeting the C-terminal sequence of the membrane-proximal ... -
Engineering pan–HIV-1 neutralization potency through multispecific antibody avidity
(National Academy of Sciences of the United States of America, 2022-01-25)Deep mining of B cell repertoires of HIV-1-infected individuals has resulted in the isolation of dozens of HIV-1 broadly neutralizing antibodies (bNAbs). Yet, it remains uncertain whether any such bNAbs alone are sufficiently ... -
Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile
; ; ; ; ; ; ; Sánchez Eugenia, Rubén; ; ; ; ; ; ; ; ; (Cell Press, 2021-09-24)
Broadly neutralizing antibodies (bnAbs) against HIV-1 are frequently associated with the presence of autoreactivity/polyreactivity, a property that can limit their use as therapeutic agents. The bnAb 4E10, targeting the ... -
HuR/ELAVL1 drives malignant peripheral nerve sheath tumor growth and metastasis
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; Izeta Permisán, Ander; ; ; ; ; ; ; Martínez Chantar, María Luz ; ; ; ; ; ; ; ; Carracedo Pérez, Arkaitz ; ; (The American Society for Clinical Investigation, 2020-07-01)
Cancer cells can develop a strong addiction to discrete molecular regulators, which control the aberrant gene expression programs that drive and maintain the cancer phenotype. Here, we report the identification of the ... -
Identification of Androgen Receptor Metabolic Correlome Reveals the Repression of Ceramide Kinase by Androgens
; ; ; ; ; ; ; ; ; ; ; ; ; Carracedo Pérez, Arkaitz (MDPI, 2021-08-26)
Prostate cancer (PCa) is one of the most prevalent cancers in men. Androgen receptor signaling plays a major role in this disease, and androgen deprivation therapy is a common therapeutic strategy in recurrent disease. ... -
Identification of proximal SUMO-dependent interactors using SUMO-ID
; ; ; ; ; ; ; ; ; Carracedo Pérez, Arkaitz; ; ; Mayor Martínez, Ugo ; ; ; (Nature Research, 2021-11-18)
[EN]The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by ... -
LUZP1 Controls Cell Division, Migration and Invasion Through Regulation of the Actin Cytoskeleton
; ; ; ; ; Rodríguez Pérez, José Antonio; Carracedo Pérez, Arkaitz ; ; (Frontiers Media, 2021-04-01)
LUZP1 is a centrosomal and actin cytoskeleton-localizing protein that regulates both ciliogenesis and actin filament bundling. As the cytoskeleton and cilia are implicated in metastasis and tumor suppression, we examined ... -
Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA
; ; ; ; ; ; ; ; ; Contreras Gómez, Xabier; ; ; ; ; ; ; (American Association for the Advancement of Science, 2021-10)
[EN]Glycolipids are prominent components of bacterial membranes that play critical roles not only in maintaining the structural integrity of the cell but also in modulating host-pathogen interactions. PatA is an essential ... -
Multiplex SERS Detection of Metabolic Alterations in Tumor Extracellular Media
; ; ; ; ; ; ; Carracedo Pérez, Arkaitz; (Wiley, 2020-04)
The composition and intercellular interactions of tumor cells in the tissues dictate the biochemical and metabolic properties of the tumor microenvironment. The metabolic rewiring has a profound impact on the properties ... -
Multivalency Transforms SARS-CoV-2 Antibodies Into Ultrapotent Neutralizers
(Nature, 2021-06-16)SARS-CoV-2, the virus responsible for COVID-19, has caused a global pandemic. Antibodies can be powerful biotherapeutics to fight viral infections. Here, we use the human apoferritin protomer as a modular subunit to drive ... -
Nanocomposite Scaffolds for Monitoring of Drug Diffusion in Three-Dimensional Cell Environments by Surface-Enhanced Raman Spectroscopy
; ; ; ; ; ; ; Carracedo Pérez, Arkaitz; (American Chemical Society, 2021-10-27)
[EN]Monitoring dynamic processes in complex cellular environments requires the integration of uniformly distributed detectors within such three-dimensional (3D) networks, to an extent that the sensor could provide real-time ... -
Neddylation inhibition ameliorates steatosis in NAFLD by boosting hepatic fatty acid oxidation via DEPTOR-mTOR axis
; ; ; ; ; ; ; ; ; ; ; ; ; Buqué García, Xabier; ; ; ; ; ; Bañales Asurmendi, Jesús María ; Ávila, Matías A. ; ; ; ; ; Mayor Martínez, Ugo ; ; ; ; ; Martínez Chantar, María Luz (Elsevier, 2021-06-18)
[EN] Objective: Neddylation is a druggable and reversible ubiquitin-like post-translational modification upregulated in many diseases, including liver fibrosis, hepatocellular carcinoma, and more recently, non-alcoholic ... -
PI3K-regulated Glycine N-methyltransferase is required for the development of prostate cancer
Zabala Letona, Amaia; Arruabarrena Aristorena, Amaia; Fernández Ruiz, Sonia; Viera, Cristina; Carlevaris, Onintza; Ercilla Eguiarte, Amaia; Mendizabal, Isabel; Martín Mateos, Teresa; Macchia, Alice; Camacho Soguero, Laura; Pujana Vaquerizo, Mikel; Sánchez Mosquera, Pilar; Torrano Moya, Verónica; Martín Martín, Natalia; Zuñiga García, Patricia; Castillo Martín, Mireia; Ugalde Olano, Aitziber; Loizaga Iriarte, Ana; Unda Urzaiz, Jesús Miguel; Mato, José M.; Berra Ramírez, Miren Edurne; Martínez Chantar, María Luz; Carracedo Pérez, Arkaitz (Springer Nature, 2022-02)
[EN] Glycine N-Methyltransferase (GNMT) is a metabolic enzyme that integrates metabolism and epigenetic regulation. The product of GNMT, sarcosine, has been proposed as a prostate cancer biomarker. This enzyme is predominantly ... -
Stromal oncostatin M cytokine promotes breast cancer progression by reprogramming the tumor microenvironment
Araujo, Angela M.; Abaurrea, Andrea; Azcoaga, Peio; López Velazco, Joanna I.; Manzano, Sara; Rodríguez Martínez, Javier; Rezola, Ricardo; Egia Mendikute, Leire; Valdés Mora, Fátima; Flores, Juana M.; Jenkins, Liam; Pulido, Laura; Osorio Querejeta, Iñaki; Fernández Nogueira, Patricia; Ferrari, Nicola; Viera, Cristina; Martín Martín, Natalia; Tzankov, Alexandar; Eppenberger Castori, Serenella; Álvarez López, Isabel; Urruticoechea, Ander; Bragado, Paloma; Coleman, Nicholas; Palazón, Asís; Carracedo Pérez, Arkaitz; Gallego Ortega, David; Calvo González, Fernando; Isacke, Clare M.; Caffarel, María M.; Lawrie, Charles (American Society of Clinical Investigation, 2022-04-01)
[EN] The tumor microenvironment (TME) is reprogrammed by cancer cells and participates in all stages of tumor progression. The contribution of stromal cells to the reprogramming of the TME is not well understood. Here, we ... -
Structural Characterization of the ICOS/ICOS-L Immune Complex Reveals High Molecular Mimicry by Therapeutic Antibodies
(Nature, 2020-10-08)The inducible co-stimulator (ICOS) is a member of the CD28/B7 superfamily, and delivers a positive co-stimulatory signal to activated T cells upon binding to its ligand (ICOS-L). Dysregulation of this pathway has been ... -
Structure of HIV-1 gp41 with its Membrane Anchors Targeted by Neutralizing Antibodies
Caillat, Christophe; Guilligay, Delphine; Torralba Iturbe, Johana; Friedrich, Nikolas; ; Trkola, Alexandra; Chipot, Christophe J.; Dehez, Francois L.; Weissenhorn, Winfried (eLife Sciences, 2021-04-19)
The HIV-1 gp120/gp41 trimer undergoes a series of conformational changes in order to catalyze gp41-induced fusion of viral and cellular membranes. Here, we present the crystal structure of gp41 locked in a fusion intermediate ... -
SUMOylation in the control of cholesterol homeostasis
Talamillo, Ana; Ajuria, Leiore; Grillo, Marco; Barroso Gomila, Orhi; Mayor Martínez, Ugo; Barrio Olano, María Rosa (The Royal Society, 2020-05)
SUMOylation-protein modification by the small ubiquitin-related modifier (SUMO)-affects several cellular processes by modulating the activity, stability, interactions or subcellular localization of a variety of substrates. ...