Cloning and expression of Hsp40 variants to study the functional association of human disaggregase

Abstract

The present project describes the cloning process of different variants of human and bacterial Hsp40s that will be used to study the functional association of the human disaggregase machinery. Different molecular biology strategies (single point mutations, domain deletion, tag insertion...) were employed to achieve this objective. All the different variants were successfully overexpressed as soluble products in a bacterial system.