Show simple item record

dc.contributor.advisorBañuelos Rodríguez, Sonia ORCID
dc.contributor.advisorMerico, Valeria
dc.contributor.authorMuguruza Montero, Arantza
dc.contributor.otherF. CIENCIA Y TECNOLOGIA
dc.contributor.otherZIENTZIA ETA TEKNOLOGIA F.
dc.date.accessioned2018-04-18T17:39:04Z
dc.date.available2018-04-18T17:39:04Z
dc.date.issued2018-04-18
dc.identifier.urihttp://hdl.handle.net/10810/26448
dc.description.abstract[EN] In vitro fertilization (IVF) is an important technique developed from assisted reproductive technologies (ARTs). Although it has been investigated since years, some optimizations should be done in order to determinate the ideal conditions for practicing this technique. In this work IVF was practiced using mice model. One million spermatozoa (sp.) per millilitre and two millions sp/mL were used in IVF in order to determinate an ideal concentration of spermatozoa for obtaining the highest fertilization rate, preimplantation developmental rate and the best blastocyst quality. Pronucleus were stained with DAPI to analyse the fertilization rate. Beside this, blastocyst were stained with DAPI after a hypotonic treatment was done to count the blastomeres for the determination of the blastocyst quality. Significant differences were not found so that using one or the other concentration we obtained similar results. However, using the lowest concentration may reduce the stress of the oocyte obtaining better result in a long-period time.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/es/
dc.subjectmouse
dc.subjectembryos
dc.subjectsperm
dc.subjectin vitro fertilization
dc.subjectblastocyst
dc.subjectpronucleus
dc.subjectDAPI
dc.subjectpreimplantation
dc.subjectfertilization rate
dc.subjecthypotonic treatment
dc.subjectIVF
dc.titleOptimization of sperm concentration for in vitro production of mouse embryoses_ES
dc.typeinfo:eu-repo/semantics/bachelorThesis
dc.date.updated2017-07-21T11:31:58Z
dc.language.rfc3066es
dc.rights.holderAtribución-CompartirIgual (cc by-sa)
dc.contributor.degreeGrado en Biotecnología;;Bioteknologiako Graduaes_ES
dc.identifier.gaurregister82122-700703-11
dc.identifier.gaurassign38476-700703


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución-CompartirIgual (cc by-sa)
Except where otherwise noted, this item's license is described as Atribución-CompartirIgual (cc by-sa)