Optimization of sperm concentration for in vitro production of mouse embryos

Abstract

[EN] In vitro fertilization (IVF) is an important technique developed from assisted reproductive technologies (ARTs). Although it has been investigated since years, some optimizations should be done in order to determinate the ideal conditions for practicing this technique. In this work IVF was practiced using mice model. One million spermatozoa (sp.) per millilitre and two millions sp/mL were used in IVF in order to determinate an ideal concentration of spermatozoa for obtaining the highest fertilization rate, preimplantation developmental rate and the best blastocyst quality. Pronucleus were stained with DAPI to analyse the fertilization rate. Beside this, blastocyst were stained with DAPI after a hypotonic treatment was done to count the blastomeres for the determination of the blastocyst quality. Significant differences were not found so that using one or the other concentration we obtained similar results. However, using the lowest concentration may reduce the stress of the oocyte obtaining better result in a long-period time.