dc.contributor.author | Rosani, Umberto | |
dc.contributor.author | Manfrin, Chiara | |
dc.contributor.author | Cajaraville Bereciartua, Miren Pilare | |
dc.contributor.author | Raccanelli, Stefano | |
dc.contributor.author | Pallavicini, Alberto | |
dc.contributor.author | Venier, Paola | |
dc.date.accessioned | 2013-05-22T11:47:21Z | |
dc.date.available | 2013-05-22T11:47:21Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | PLoS ONE 8(1) : (2013) // e54602 | es |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/10143 | |
dc.description.abstract | [ENG]Aiming at an integrated and mechanistic view of the early biological effects of selected metals in the marine sentinel
organism Mytilus galloprovincialis, we exposed mussels for 48 hours to 50, 100 and 200 nM solutions of equimolar Cd, Cu
and Hg salts and measured cytological and molecular biomarkers in parallel. Focusing on the mussel gills, first target of toxic
water contaminants and actively proliferating tissue, we detected significant dose-related increases of cells with micronuclei and other nuclear abnormalities in the treated mussels, with differences in the bioconcentration of the three metals determined in the mussel flesh by atomic absorption spectrometry. Gene expression profiles, determined in the same
individual gills in parallel, revealed some transcriptional changes at the 50 nM dose, and substantial increases of differentially expressed genes at the 100 and 200 nM doses, with roughly similar amounts of up- and down-regulated genes. The functional annotation of gill transcripts with consistent expression trends and significantly altered at least in one
dose point disclosed the complexity of the induced cell response. The most evident transcriptional changes concerned protein synthesis and turnover, ion homeostasis, cell cycle regulation and apoptosis, and intracellular trafficking (transcript
sequences denoting heat shock proteins, metal binding thioneins, sequestosome 1 and proteasome subunits, and GADD45 exemplify up-regulated genes while transcript sequences denoting actin, tubulins and the apoptosis inhibitor 1 exemplify
down-regulated genes). Overall, nanomolar doses of co-occurring free metal ions have induced significant structural and
functional changes in the mussel gills: the intensity of response to the stimulus measured in laboratory supports the
additional validation of molecular markers of metal exposure to be used in Mussel Watch programs | es |
dc.description.sponsorship | Work granted by MIUR and Co.Ri.La. to PV. LV is currently supported by the FP7-KBBE-2010-4-266157 Bivalife project. Work in the laboratory of MPC is
funded by grants to consolidated research groups (ref GIC07/26-IT-393-07, Basque Government), and to the unit of formation and research (UFI ref 11/37,
University of the Basque Country) and projects Nanoretox (ref CP-FP 214478-2, UE 7th FP) and Nanocancer (ref CTM2009-13477, Spanish Ministry of Science and
Innovation) | es |
dc.language.iso | eng | es |
dc.publisher | PloS ONE | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | in situ-hybridization | en |
dc.subject | gene-expression | en |
dc.subject | oxidative stress | en |
dc.subject | heavy-metals | en |
dc.subject | metallothionein genes | en |
dc.subject | digestive gland | en |
dc.subject | blue mussel | en |
dc.title | DNA Damage and Transcriptional Changes in the Gills of Mytilus galloprovincialis Exposed to Nanomolar Doses of Combined Metal Salts (Cd, Cu, Hg) | en |
dc.type | info:eu-repo/semantics/article | en |
dc.rights.holder | 2013 Varotto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited | es |
dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054602 | es |
dc.departamentoes | Zoología y biología celular animal | es_ES |
dc.departamentoeu | Zoologia eta animalia zelulen biologia | es_ES |
dc.subject.categoria | AGRICULTURAL AND BIOLOGICAL SCIENCES | |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | BIOCHEMISTRY AND MOLECULAR BIOLOGY | |