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dc.contributor.authorSan Millán Gutiérrez, Rosario María
dc.contributor.authorMartínez Ballesteros, Ilargi
dc.contributor.authorRementeria Ruiz, Aitor Domingo
dc.contributor.authorGaraizar Candina, Javier
dc.contributor.authorBikandi Bikandi, Joseba
dc.date.accessioned2014-01-09T13:05:32Z
dc.date.available2014-01-09T13:05:32Z
dc.date.issued2013-12-05
dc.identifier.citationBMC Research Notes 6(513) : (2013)es
dc.identifier.issn1756-0500
dc.identifier.urihttp://hdl.handle.net/10810/11186
dc.description.abstract[EN] Background: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism of PCR products (PCR-RFLP) are extensively used molecular biology techniques. An exercise for the design and simulation of PCR and PCR-RFLP experiments will be a useful educational tool. Findings: An online PCR and PCR-RFLP exercise has been create that requires users to find the target genes,compare them, design primers, search for restriction endonucleases, and finally to simulate the experiment. Each user of the service is randomly assigned a gene from Escherichia coli; to complete the exercise, users must design an experiment capable of distinguishing among E. coli strains. By applying the experimental procedure to all completely sequenced E. coli, a basic understanding of strain comparison and clustering can also be acquired. Comparison of results obtained in different experiments is also very instructive. Conclusions: The exercise is freely available at http://insilico.ehu.es/edu.es
dc.language.isoenges
dc.publisherBioMed Centrales
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectPCRes
dc.subjectPCR-RFLPes
dc.subjectexercisees
dc.subjectprimeres
dc.subjectrestriction endonucleasees
dc.titleOnline exercise for the design and simulation of PCR and PCR-RFLP experimentses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2013 San Millán et al.; licensee BioMed Central Ltd.This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://www.biomedcentral.com/1756-0500/6/513es
dc.identifier.doidoi:10.1186/1756-0500-6-513
dc.departamentoesInmunología, microbiología y parasitologíaes_ES
dc.departamentoeuImmunologia, mikrobiologia eta parasitologiaes_ES
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY
dc.subject.categoriaMEDICINE


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