dc.contributor.author | Axpe Iza, Eneko | |
dc.contributor.author | López Euba, Tamara | |
dc.contributor.author | Castellanos Rubio, Ainara | |
dc.contributor.author | Mérida Sanz, David | |
dc.contributor.author | García Martínez, José Angel | |
dc.contributor.author | Plaza-Izurieta, Leticia | |
dc.contributor.author | Fernández-Jiménez, Nora | |
dc.contributor.author | Plazaola Muguruza, Fernando | |
dc.contributor.author | Bilbao Catalá, José Ramón | |
dc.date.accessioned | 2014-01-09T19:03:05Z | |
dc.date.available | 2014-01-09T19:03:05Z | |
dc.date.issued | 2014-01-02 | |
dc.identifier.citation | PLoS ONE 9(1) : (2014) // e83838 | es |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/11188 | |
dc.description | 5 p. | es |
dc.description.abstract | Positron annihilation lifetime spectroscopy (PALS) provides a direct measurement of the free volume void sizes in polymers and biological systems. This free volume is critical in explaining and understanding physical and mechanical properties of polymers. Moreover, PALS has been recently proposed as a potential tool in detecting cancer at early stages, probing the differences in the subnanometer scale free volume voids between cancerous/healthy skin samples of the same patient. Despite several investigations on free volume in complex cancerous tissues, no positron annihilation studies of living cancer cell cultures have been reported. We demonstrate that PALS can be applied to the study in human living 3D cell cultures. The technique is also capable to detect atomic scale changes in the size of the free volume voids due to the biological responses to TGF-β. PALS may be developed to characterize the effect of different culture conditions in the free volume voids of cells grown in vitro. | es |
dc.description.sponsorship | Basque Department of Industry grant no. SAI11/263 – PRODETEC and Basque Department of Education, Universities and Research grant no. IT/443/10. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | absorption spectroscopy | es |
dc.subject | adenocarcinomas | es |
dc.subject | cell cultures | es |
dc.subject | cell differentiation | es |
dc.subject | collagens | es |
dc.subject | epithelial cells | es |
dc.subject | immunofluorescence | es |
dc.subject | polymers | es |
dc.title | Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | © 2014 Axpe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | es |
dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0083838 | es |
dc.identifier.doi | 10.1371/journal.pone.0083838 | |
dc.departamentoes | Pediatría | es_ES |
dc.departamentoes | Física aplicada II | es_ES |
dc.departamentoes | Genética, antropología física y fisiología animal | es_ES |
dc.departamentoes | Electricidad y electrónica | es_ES |
dc.departamentoeu | Pediatria | es_ES |
dc.departamentoeu | Fisika aplikatua II | es_ES |
dc.departamentoeu | Genetika,antropologia fisikoa eta animalien fisiologia | es_ES |
dc.departamentoeu | Elektrizitatea eta elektronika | es_ES |
dc.subject.categoria | AGRICULTURAL AND BIOLOGICAL SCIENCES | |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | BIOCHEMISTRY AND MOLECULAR BIOLOGY | |