dc.contributor.author | Eseberri Barace, Itziar | |
dc.contributor.author | Lasa Elguezua, Arrate | |
dc.contributor.author | Churruca Ortega, Itziar | |
dc.contributor.author | Portillo Baquedano, María Puy | |
dc.date.accessioned | 2014-01-24T18:50:00Z | |
dc.date.available | 2014-01-24T18:50:00Z | |
dc.date.issued | 2013-05 | |
dc.identifier.citation | PLoS One 8(5) : (2013) // e63918 | es |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/11278 | |
dc.description.abstract | Objective: Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue.
Methods: 3T3-L1 maturing pre-adipocytes were treated during differentiation with 25 mu M of resveratrol or with its metabolites and 3T3-L1 mature adipocytes were treated for 24 hours with 10 mM resveratrol or its metabolites. The gene expression of adiponectin, leptin, visfatin and apelin was assessed by Real Time RT-PCR and their concentration in the incubation medium was quantified by ELISA.
Results: Resveratrol reduced mRNA levels of leptin and increased those of adiponectin. It induced the same changes in leptin secretion. Trans-resveratrol-3-O-glucuronide and trans-resveratrol-4'-O-glucuronide increased apelin and visfatin mRNA levels. Trans-resveratrol-3-O-sulfate reduced leptin mRNA levels and increased those of apelin and visfatin.
Conclusions: The present study shows for the first time that resveratrol metabolites have a regulatory effect on adipokine expression and secretion. Since resveratrol has been reported to reduce body-fat accumulation and to improve insulin sensitivity, and considering that these effects are mediated in part by changes in the analyzed adipokines, it may be proposed that resveratrol metabolites play a part in these beneficial effects of resveratrol. | es |
dc.description.sponsorship | This study was supported by grants from the Ministerio de Economia y Competitividad (AGL2011-27406-ALI), Instituto de Salud Carlos III (RETIC PREDIMED and CiberObn), Government of the Basque Country (IT-512-13) and University of the Basque Country (UPV/EHU) (ELDUNANOTEK UFI11/32). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation | info:eu-repo/grantAgreement/MINECO/AGL2011-27406-ALI | |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | activated protein kinase | es |
dc.subject | insulin resistance | es |
dc.subject | gene expression | es |
dc.subject | low bioavailability | es |
dc.subject | skeletal muscle | es |
dc.subject | adipose tissue | es |
dc.subject | mice | es |
dc.subject | rats | es |
dc.subject | apelin | es |
dc.subject | leptin | es |
dc.title | Resveratrol Metabolites Modify Adipokine Expression and Secretion in 3T3-L1 Pre-Adipocytes and Mature Adipocytes | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | © 2013 Eseberri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | es |
dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063918 | es |
dc.identifier.doi | 10.1371/journal.pone.0063918 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |
dc.subject.categoria | AGRICULTURAL AND BIOLOGICAL SCIENCES | |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | BIOCHEMISTRY AND MOLECULAR BIOLOGY | |