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dc.contributor.authorEseberri Barace, Itziar
dc.contributor.authorLasa Elguezua, Arrate
dc.contributor.authorChurruca Ortega, Itziar
dc.contributor.authorPortillo Baquedano, María Puy
dc.date.accessioned2014-01-24T18:50:00Z
dc.date.available2014-01-24T18:50:00Z
dc.date.issued2013-05
dc.identifier.citationPLoS One 8(5) : (2013) // e63918es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/11278
dc.description.abstractObjective: Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue. Methods: 3T3-L1 maturing pre-adipocytes were treated during differentiation with 25 mu M of resveratrol or with its metabolites and 3T3-L1 mature adipocytes were treated for 24 hours with 10 mM resveratrol or its metabolites. The gene expression of adiponectin, leptin, visfatin and apelin was assessed by Real Time RT-PCR and their concentration in the incubation medium was quantified by ELISA. Results: Resveratrol reduced mRNA levels of leptin and increased those of adiponectin. It induced the same changes in leptin secretion. Trans-resveratrol-3-O-glucuronide and trans-resveratrol-4'-O-glucuronide increased apelin and visfatin mRNA levels. Trans-resveratrol-3-O-sulfate reduced leptin mRNA levels and increased those of apelin and visfatin. Conclusions: The present study shows for the first time that resveratrol metabolites have a regulatory effect on adipokine expression and secretion. Since resveratrol has been reported to reduce body-fat accumulation and to improve insulin sensitivity, and considering that these effects are mediated in part by changes in the analyzed adipokines, it may be proposed that resveratrol metabolites play a part in these beneficial effects of resveratrol.es
dc.description.sponsorshipThis study was supported by grants from the Ministerio de Economia y Competitividad (AGL2011-27406-ALI), Instituto de Salud Carlos III (RETIC PREDIMED and CiberObn), Government of the Basque Country (IT-512-13) and University of the Basque Country (UPV/EHU) (ELDUNANOTEK UFI11/32). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relationinfo:eu-repo/grantAgreement/MINECO/AGL2011-27406-ALI
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectactivated protein kinasees
dc.subjectinsulin resistancees
dc.subjectgene expressiones
dc.subjectlow bioavailabilityes
dc.subjectskeletal musclees
dc.subjectadipose tissuees
dc.subjectmicees
dc.subjectratses
dc.subjectapelines
dc.subjectleptines
dc.titleResveratrol Metabolites Modify Adipokine Expression and Secretion in 3T3-L1 Pre-Adipocytes and Mature Adipocyteses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2013 Eseberri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063918es
dc.identifier.doi10.1371/journal.pone.0063918
dc.departamentoesFarmacia y ciencias de los alimentoses_ES
dc.departamentoeuFarmazia eta elikagaien zientziakes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaMEDICINE
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY


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