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dc.contributor.authorLopes, Bruno S.
dc.contributor.authorGallego Andrés, Lucía ORCID
dc.contributor.authorAmyes, Sebastian G. B.
dc.date.accessioned2014-02-04T15:29:02Z
dc.date.available2014-02-04T15:29:02Z
dc.date.issued2013-04
dc.identifier.citationJournal of Infection in Developing Countries 7(4) : 323-328 (2013)es
dc.identifier.issn1972-2680
dc.identifier.urihttp://hdl.handle.net/10810/11335
dc.description.abstractIntroduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) gene caused over-expression of the latter and cephalosporin resistance; mutations in the gyrA(Ser83 to Leu) and parC (Ser-80 to Phe) genes were commensurate with fluoroquinolone resistance. In addition, the adeA, adeB efflux genes were over-expressed. All 15 isolates were positive for at least two aminoglycoside resistance genes. Conclusion: This is one of the first reports analyzing the multi-drug resistance profile of A. baumannii strains isolated in Bolivia and shows that the over-expression of thebla(OXA-58), bla(ADC) and efflux genes together with aminoglycoside modifying enzymes and mutations in DNA topoisomerases are responsible for the multi-resistance of the bacteria and the subsequent difficulty in treating infections caused by them.es
dc.description.sponsorshipBSL was funded by the University of Edinburgh Overseas Research scholarship. A part of this project was funded by an RA0119 MRC grant. LG was funded by a grant from Vicerrectorado de Investigacion de la Universidad del Pais Vasco (Plan de movilidad de investigadores)es
dc.language.isoenges
dc.publisherJournal of Infection in Developing Countrieses
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAcinetobacter baumanniies
dc.subjectinsertion sequenceses
dc.subjectbeta-lactamaseses
dc.subjectcarbapenemses
dc.subjectgenees
dc.subjectenvironmentes
dc.titleMulti-drug resistance profiles and the genetic features of Acinetobacter baumannii isolates from Boliviaes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2013 Lopes et al. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.es
dc.relation.publisherversionhttp://www.jidc.org/index.php/journal/article/view/23592642es
dc.identifier.doi10.3855/jidc.2711
dc.departamentoesInmunología, microbiología y parasitologíaes_ES
dc.departamentoeuImmunologia, mikrobiologia eta parasitologiaes_ES
dc.subject.categoriaINFECTIOUS DISEASES
dc.subject.categoriaVIROLOGY
dc.subject.categoriaPARASITOLOGY
dc.subject.categoriaMICROBIOLOGY


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