Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Querido, Jailson F. B.
Martí, Gerardo A.
Guerín Aguilar, Diego Marcelo A.
Silva, Marcelo Sousa
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BioMed Research International 2013 : (2013) // Article ID 218593
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.