Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Querido, Jailson F. B.
Martí, Gerardo A.
Guerín Aguilar, Diego Marcelo A.
Silva, Marcelo Sousa
MetadatosMostrar el registro completo del ítem
BioMed Research International 2013 : (2013) // Article ID 218593
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.