dc.contributor.author | Blanco, M. Dolores | |
dc.contributor.author | Guerrero, Sandra | |
dc.contributor.author | Benito, Marta | |
dc.contributor.author | Fernández, Ana | |
dc.contributor.author | Teijón, César | |
dc.contributor.author | Olmo, Rosa | |
dc.contributor.author | Katime Amashta, Issa Antonio | |
dc.contributor.author | Teijón, José M. | |
dc.date.accessioned | 2014-02-20T14:36:03Z | |
dc.date.available | 2014-02-20T14:36:03Z | |
dc.date.issued | 2011-09 | |
dc.identifier.citation | Polymers 3(3) : 1107-1125 (2011) | es |
dc.identifier.issn | 2073-4360 | |
dc.identifier.uri | http://hdl.handle.net/10810/11587 | |
dc.description.abstract | Folate-targeted poly[(p-nitrophenyl acrylate)-co-(N-isopropylacrylamide)] nanohydrogel (F-SubMG) was loaded with 5-fluorouracil (5-FU) to obtain low (16.3 +/- 1.9 mu g 5-FU/mg F-SubMG) and high (46.8 +/- 3.8 mu g 5-FU/mg F-SubMG) load 5-FU-loaded F-SubMGs. The complete in vitro drug release took place in 8 h. The cytotoxicity of unloaded F-SubMGs in MCF7 and HeLa cells was low; although it increased for high F-SubMG concentration. The administration of 10 mu M 5-FU by 5-FU-loaded F-SubMGs was effective on both cellular types. Cell uptake of F-SubMGs took place in both cell types, but it was higher in HeLa cells because they are folate receptor positive. After subcutaneous administration (28 mg 5-FU/kg b.w.) in Wistar rats, F-SubMGs were detected at the site of injection under the skin. Histological studies indicated that the F-SubMGs were surrounded by connective tissue, without any signs of rejections, even 60 days after injection. Pharmacokinetic study showed an increase in MRT (mean residence time) of 5-FU when the drug was administered by drug-loaded F-SubMGs. | es |
dc.description.sponsorship | The financial support from the Ministerio de Ciencia e Innovacion of Spain (MAT 2010-21509-CO3-02) and the UCM-CAM for Consolidated Research Groups (Group 920613) is gratefully acknowledged. The authors are grateful to von Kobbe (Chimera Pharma of Bionostra Group) and Tierrez (Centro Nacional de Investigaciones Cardiovasculares, CNIC, Spain) for the gift of MCF7 and HeLa cell lines. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation | info:eu-repo/grantAgreement/MICINN/MAT2010-21509-CO3-02 | |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | drug delivery | es |
dc.subject | folate conjugate nanogel | es |
dc.subject | 5-fluorouracil | es |
dc.subject | culture cells | es |
dc.subject | pharmacokinetic | es |
dc.title | In Vitro and In Vivo Evaluation of a Folate-Targeted Copolymeric Submicrohydrogel Based on N-Isopropylacrylamide as 5-Fluorouracil Delivery System | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article
distributed under the terms and conditions of the Creative Commons Attribution license
(http://creativecommons.org/licenses/by/3.0/). | es |
dc.relation.publisherversion | http://www.mdpi.com/2073-4360/3/3/1107 | es |
dc.identifier.doi | 10.3390/polym3031107 | |
dc.departamentoes | Química física | es_ES |
dc.departamentoeu | Kimika fisikoa | es_ES |
dc.subject.categoria | CHEMISTRY, MATERIALS | |