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dc.contributor.authorTeifoori, Fardis
dc.contributor.authorShams-Ghahfarokhi, Masoomeh
dc.contributor.authorPostigo Resa, Idoya
dc.contributor.authorRazzaghi-Abyaneh, Mehdi
dc.contributor.authorEslamifar, Ali
dc.contributor.authorGutiérrez Rodríguez, Antonio
dc.contributor.authorSuñén Pardo, María Ester
dc.contributor.authorMartínez Quesada, Jorge
dc.date.accessioned2015-11-24T14:02:49Z
dc.date.available2015-11-24T14:02:49Z
dc.date.issued2014-08-05
dc.identifier.citationAllergy, Asthma & Clinical Immunology 10 : (2014) // Article ID 41es
dc.identifier.issn1710-1484
dc.identifier.urihttp://hdl.handle.net/10810/16168
dc.description.abstractBackground: There has been a significant growth in the prevalence of allergy, mainly associated to IgE-mediated disorders such as asthma and rhinitis. The identification of atopy in asthmatic patients through the measurement of specific IgE can help to identify risk factors that cause asthmatic symptoms in patients. The development and use of individualized allergen-based tests by the Component Resolved Diagnosis has been a crucial advance in the accurate diagnosis and control of allergic patients. The objective of this work was to assess the usefulness of molecular diagnosis to identify environmental allergens as possible factors influencing the development and manifestation of asthma in a group of asthmatic patients from Iran. Methods: Studied population: 202 adult asthmatic patients treated at the Loghman Hakim Hospital and Pasteur Institute of Teheran (Iran) from 2011 to 2012. Specific IgE determined by the ImmunoCAP system were used to both evaluate the patients' atopic condition and the molecules involved in the allergic sensitization. SDS-PAGE IgE-immunoblotting associated with mass spectrometry was carried out to study the cockroach IgE-binding sensitizing proteins. Results: Forty-five percent of all patients could be considered atopic individuals. Eighty-two percent of atopic patients were sensitized to pollen allergens. The Salsola kali (Sal k 1) and the Phleum pratense (rPhl p 1 and/or rPhl p 5) major allergens were the most common sensitizers among pollens (71% and 18%, respectively). Thirty-five percent of the atopic population was sensitized to cockroach. Four different allergens, including a previously unknown alpha-amylase, were identified in the cockroach extract. No significant associations could be demonstrated between the severity of asthma and the specific IgE levels in the atopic population. Statistical analysis identified the Sal k 1 as the main protein allergen influencing the development and expression of asthma in the studied population. Conclusions: Pollen and cockroach were the most relevant allergen sources in the asthmatic population. The Salsola kali major allergen was the main cause for sensitization in the atopic patients suffering asthma. Using the Component Resolved Diagnosis, it was possible to identify a new Blattella germanica cockroach allergen (Blattella alpha amylase 53 kDa) that could sensitize a relevant percentage of this population.es
dc.description.sponsorshipThis study has been founded by the Government of The Basque Country, Project IT787-13.es
dc.language.isoenges
dc.publisherBiomed Centrales
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectallergyes
dc.subjectatopyes
dc.subjectspecific IgEes
dc.subjectcomponent resolved diagnosises
dc.subjectasthmaes
dc.subjectrisk factores
dc.subjectpollenes
dc.subjectmouldes
dc.subjectcockroaches
dc.subjectproteines
dc.subjectallergenes
dc.subjectcommunity respiratory healthes
dc.subjectaeroallergenses
dc.subjectIgEes
dc.subjectnitrocellulosees
dc.subjectpolyacrylamidees
dc.subjectimmunotherapyes
dc.subjectreactivityes
dc.subjectcockroaches
dc.titleIdentification of the main allergen sensitizers in an Iran asthmatic population by molecular diagnosises
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2014 Teifoori et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.es
dc.relation.publisherversionhttp://www.aacijournal.com/content/10/1/41/abstractes
dc.identifier.doi10.1186/1710-1492-10-41
dc.departamentoesInmunología, microbiología y parasitologíaes_ES
dc.departamentoeuImmunologia, mikrobiologia eta parasitologiaes_ES
dc.subject.categoriaIMMUNOLOGY AND ALLERGY
dc.subject.categoriaPULMONARY AND RESPIRATORY MEDICINE


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