dc.contributor.author | Bustillo Zabalbeitia, Itsasne | |
dc.contributor.author | Montessuit, Sylvie | |
dc.contributor.author | Raemy, Etienne | |
dc.contributor.author | Basáñez Asúa, Gorka | |
dc.contributor.author | Terrones Urio, Oihana | |
dc.contributor.author | Martinou, Jean-Claude | |
dc.date.accessioned | 2015-12-09T17:00:12Z | |
dc.date.available | 2015-12-09T17:00:12Z | |
dc.date.issued | 2014-07-18 | |
dc.identifier.citation | PLOS ONE 9 (7) : (2014) // Article ID e102738 | es |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10810/16405 | |
dc.description.abstract | Dynamin-Related Protein 1 (Drp1), a large GTPase of the dynamin superfamily, is required for mitochondrial fission in healthy and apoptotic cells. Drp1 activation is a complex process that involves translocation from the cytosol to the mitochondrial outer membrane (MOM) and assembly into rings/spirals at the MOM, leading to membrane constriction/division. Similar to dynamins, Drp1 contains GTPase (G), bundle signaling element (BSE) and stalk domains. However, instead of the lipid-interacting Pleckstrin Homology (PH) domain present in the dynamins, Drp1 contains the so-called B insert or variable domain that has been suggested to play an important role in Drp1 regulation. Different proteins have been implicated in Drp1 recruitment to the MOM, although how MOM-localized Drp1 acquires its fully functional status remains poorly understood. We found that Drp1 can interact with pure lipid bilayers enriched in the mitochondrion-specific phospholipid cardiolipin (CL). Building on our previous study, we now explore the specificity and functional consequences of this interaction. We show that a four lysine module located within the B insert of Drp1 interacts preferentially with CL over other anionic lipids. This interaction dramatically enhances Drp1 oligomerization and assembly-stimulated GTP hydrolysis. Our results add significantly to a growing body of evidence indicating that CL is an important regulator of many essential mitochondrial functions. | es |
dc.description.sponsorship | This work was funded by the Swiss National Science Foundation (31993A-141068/1), IGE3 and the State of Geneva (J.-C.M.), the Spanish Ministerio de Ciencia e Innovacion grant BFU2011-28566 and the Basque Government grant IT838-13 (G.B., O.T.). O.T. was supported by a postdoctoral Juan de la Cierva fellow, Spanish Government, and by a FEBS Short-Term fellowship. I.B.-Z. was supported by a predoctoral fellowship from the Basque Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library Science | es |
dc.relation | info:eu-repo/grantAgreement/MINECO/BFU2011-28566 | |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | dominant optic atrophy | es |
dc.subject | mitochondrial fission | es |
dc.subject | oxidative-phosphorylation | es |
dc.subject | conformational-changes | es |
dc.subject | menbrane-binding | es |
dc.subject | mammalian cells | es |
dc.subject | DRP1 | es |
dc.subject | GTPase | es |
dc.subject | fusion | es |
dc.subject | domain | es |
dc.title | Specific Interaction with Cardiolipin Triggers Functional Activation of Dynamin-Related Protein 1 | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | 2014 Bustillo-Zabalbeitia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which
permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | es |
dc.relation.publisherversion | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102738#abstract0 | es |
dc.identifier.doi | 10.1371/journal.pone.0102738 | |
dc.departamentoes | Bioquímica y biología molecular | es_ES |
dc.departamentoeu | Biokimika eta biologia molekularra | es_ES |
dc.subject.categoria | AGRICULTURAL AND BIOLOGICAL SCIENCES | |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | BIOCHEMISTRY AND MOLECULAR BIOLOGY | |