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dc.contributor.authorBlanco Criado, Lorena
dc.contributor.authorSanz Echevarría, María Begoña
dc.contributor.authorPérez Urzelai, Itxaro
dc.contributor.authorSánchez Fernández, Clara Eugenia
dc.contributor.authorCándenas, María Luz
dc.contributor.authorPinto, Francisco M.
dc.contributor.authorGil Goicouría, Francisco Javier
dc.contributor.authorCasis Sáenz, Luis
dc.contributor.authorLópez Fernández de Villaverde, José Ignacio
dc.contributor.authorLarrinaga Embeita, Gorka
dc.date.accessioned2016-01-21T15:22:59Z
dc.date.available2016-01-21T15:22:59Z
dc.date.issued2014-05-30
dc.identifier.citationBMC Cancer 14 : (2014) // Article ID 386es
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/10810/16788
dc.description.abstractBackground: Advances in the knowledge of renal neoplasms have demonstrated the implication of several proteases in their genesis, growth and dissemination. Glutamyl-aminopeptidase (GAP) (EC. 3.4.11.7) is a zinc metallopeptidase with angiotensinase activity highly expressed in kidney tissues and its expression and activity have been associated wtih tumour development. Methods: In this prospective study, GAP spectrofluorometric activity and immunohistochemical expression were analysed in clear-cell (CCRCC), papillary (PRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytoma (RO). Data obtained in tumour tissue were compared with those from the surrounding uninvolved kidney tissue. In CCRCC, classic pathological parameters such as grade, stage and tumour size were stratified following GAP data and analyzed for 5-year survival. Results: GAP activity in both the membrane-bound and soluble fractions was sharply decreased and its immunohistochemical expression showed mild staining in the four histological types of renal tumours. Soluble and membrane-bound GAP activities correlated with tumour grade and size in CCRCCs. Conclusions: This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms.es
dc.description.sponsorshipWe wish to thank Arantza Perez (UPV/EHU) for her technical contribution to this study. The authors also thank Ana Abascal, Alicia Esteve and Mar Gonzalez, lab technicians at the Department of Pathology, Cruces University Hospital, for their excellent immunohistochemical work. This work was supported by grants from the Basque Government (IT8-11/13), the University of the Basque Country UPV/EHU (UFI 11/44, EHUA 12/15) and the Gangoiti Barrera Foundation. We want to particularly acknowledge the patients enrolled in this study for their participation and the Basque Biobank for Research-OEHUN for this collaboration.es
dc.language.isoenges
dc.publisherBiomed Centrales
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectglutamyl-aminopeptidasees
dc.subjectaminopeptidase Aes
dc.subjectangiotensinasees
dc.subjectangiotensines
dc.subjectclear cell renal cell carcinomaes
dc.subjectrenal neoplasmes
dc.subjectrenin-angiotension systemes
dc.subjectreal-time PCRes
dc.subjectcell carcinomaes
dc.subjecttachykinin receptorses
dc.subjectcanceres
dc.subjectkidneyes
dc.subjectectopeptidaseses
dc.subjecthypothesises
dc.subjectIV/CD26es
dc.subjecttissuees
dc.titleAltered glutamyl-aminopeptidase activity and expression in renal neoplasmses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2014 Blanco et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.es
dc.relation.publisherversionhttp://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-386#Abs1es
dc.identifier.doi10.1186/1471-2407-14-386
dc.departamentoesEnfermeríaes_ES
dc.departamentoesFisiologíaes_ES
dc.departamentoeuErizaintzaes_ES
dc.departamentoeuFisiologiaes_ES
dc.subject.categoriaGENETICS AND HEREDITY
dc.subject.categoriaONCOLOGY


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