UPV-EHU ADDI
  • Back
    • English
    • Español
    • Euskera
  • Login
  • English 
    • English
    • Español
    • Euskera
  • FAQ
View Item 
  •   Home
  • INVESTIGACIÓN
  • Artículos, Comunicaciones, Libros
  • Artículos
  • View Item
  •   Home
  • INVESTIGACIÓN
  • Artículos, Comunicaciones, Libros
  • Artículos
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

TWEAK/Fn14 Signaling Is Required for Liver Regeneration after Partial Hepatectomy in Mice

Thumbnail
View/Open
journal.pone.0083987.PDF (14.54Mb)
Date
2014-01-09
Author
Karaca, Gamze
Swiderska-Syn, Marzena
Xie, Guanhua
Syn, Wing-Kin
Krüger, Leandi
Verdelho Machado, Mariana
Garman, Katherine
Choi, Steve S.
Michelotti, Gregory A.
Burkly, Linda C.
Ochoa Olascoaga, Begoña
Diehl, Anna Mae
Metadata
Show full item record
PLOS ONE 9(1) : (2014) // Article ID e83987
URI
http://hdl.handle.net/10810/17469
Abstract
Background & Aims: Pro-inflammatory cytokines are important for liver regeneration after partial hepatectomy (PH). Expression of Fibroblast growth factor-inducible 14 (Fn14), the receptor for TNF-like weak inducer of apoptosis (TWEAK), is induced rapidly after PH and remains elevated throughout the period of peak hepatocyte replication. The role of Fn14 in post-PH liver regeneration is uncertain because Fn14 is expressed by liver progenitors and TWEAK-Fn14 interactions stimulate progenitor growth, but replication of mature hepatocytes is thought to drive liver regeneration after PH. Methods: To clarify the role of TWEAK-Fn14 after PH, we compared post-PH regenerative responses in wild type (WT) mice, Fn14 knockout (KO) mice, TWEAK KO mice, and WT mice treated with anti-TWEAK antibodies. Results: In WT mice, rare Fn14(+) cells localized with other progenitor markers in peri-portal areas before PH. PH rapidly increased proliferation of Fn14(+) cells; hepatocytic cells that expressed Fn14 and other progenitor markers, such as Lgr5, progressively accumulated from 12-8 h post-PH and then declined to baseline by 96 h. When TWEAK/Fn14 signaling was disrupted, progenitor accumulation, induction of pro-regenerative cytokines, hepatocyte and cholangiocyte proliferation, and over-all survival were inhibited, while post-PH liver damage and bilirubin levels were increased. TWEAK stimulated proliferation and increased Lgr5 expression in cultured liver progenitors, but had no effect on either parameter in cultured primary hepatocytes. Conclusions: TWEAK-FN14 signaling is necessary for the healthy adult liver to regenerate normally after acute partial hepatectomy.
Collections
  • Artículos

DSpace software copyright © 2002-2015  DuraSpace
OpenAIRE
OpenAIRE
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesDepartamentos (cas.)Departamentos (eus.)SubjectsThis CollectionBy Issue DateAuthorsTitlesDepartamentos (cas.)Departamentos (eus.)Subjects

My Account

Login

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
OpenAIRE
OpenAIRE