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dc.contributor.authorLarrinaga Embeita, Gorka ORCID
dc.contributor.authorPérez Urzelai, Itxaro ORCID
dc.contributor.authorSanz Echevarría, María Begoña
dc.contributor.authorBeitia San Vicente, Maider
dc.contributor.authorErrarte Yarza, Peio
dc.contributor.authorFernández Atucha, Ainhoa
dc.contributor.authorBlanco Criado, Lorena
dc.contributor.authorEtxezarraga Zuluaga, María Carmen
dc.contributor.authorGil Goicouría, Francisco Javier ORCID
dc.contributor.authorLópez Fernández de Villaverde, José Ignacio ORCID
dc.date.accessioned2016-05-02T15:38:38Z
dc.date.available2016-05-02T15:38:38Z
dc.date.issued2015-03-19
dc.identifier.citationPlos One 10(3) : (2015) // Article ID e0119436es
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10810/18143
dc.description.abstractBackground Dipeptidyl-peptidase IV (EC 3.4.14.5) (DPPIV) is a serine peptidase involved in cell differentiation, adhesion, immune modulation and apoptosis, functions that control neoplastic transformation. Previous studies have demonstrated altered expression and activity of tissue and circulating DPPIV in several cancers and proposed its potential usefulness for early diagnosis in colorectal cancer (CRC). Methods and principal findings The activity and mRNA and protein expression of DPPIV was prospectively analyzed in adenocarcinomas, adenomas, uninvolved colorectal mucosa and plasma from 116 CRC patients by fluorimetric, quantitative RT-PCR and immunohistochemical methods. Results were correlated with the most important classic pathological data related to aggressiveness and with 5-year survival rates. Results showed that: 1) mRNA levels and activity of DPPIV increased in colorectal neoplasms (Kruskal-Wallis test, p<0.01); 2) Both adenomas and CRCs displayed positive cytoplasmic immunostaining with luminal membrane reinforcement; 3) Plasmatic DPPIV activity was lower in CRC patients than in healthy subjects (Mann-U test, p<0.01); 4) Plasmatic DPPIV activity was associated with worse overall and disease-free survivals (log-rank p<0.01, Cox analysis p<0.01). Conclusion/significance 1) Up-regulation of DPPIV in colorectal tumors suggests a role for this enzyme in the neoplastic transformation of colorectal tissues. This finding opens the possibility for new therapeutic targets in these patients. 2) Plasmatic DPPIV is an independent prognostic factor in survival of CRC patients. The determination of DPPIV activity levels in the plasma may be a safe, minimally invasive and inexpensive way to define the aggressiveness of CRC in daily practice.es
dc.description.sponsorshipThis work was supported by grants from the Basque Government (IT8-11/13), the University of the Basque Country UPV/EHU (UFI 11/44), and the Gangoiti Barrera Foundation. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.es
dc.language.isoenges
dc.publisherPublic Library Sciencees
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectfibroblast activation proteines
dc.subjectcarcinoma-associated fibroblastses
dc.subjectcolon-canceres
dc.subjectaminopeptidase N/CD13es
dc.subjectserum CD26es
dc.subjectexpressiones
dc.subjectmarkerses
dc.subjectimpactes
dc.titleDipeptidyl-Peptidase IV Activity Is Correlated with Colorectal Cancer Prognosises
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2015 Larrinaga et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedes
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119436#abstract0es
dc.identifier.doi10.1371/journal.pone.0119436
dc.departamentoesEnfermeríaes_ES
dc.departamentoesEspecialidades médico-quirúrgicases_ES
dc.departamentoesFisiologíaes_ES
dc.departamentoeuErizaintzaes_ES
dc.departamentoeuMedikuntza eta kirurgia espezialitateakes_ES
dc.departamentoeuFisiologiaes_ES
dc.subject.categoriaAGRICULTURAL AND BIOLOGICAL SCIENCES
dc.subject.categoriaBIOCHEMISTRY AND MOLECULAR BIOLOGY
dc.subject.categoriaMEDICINE


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