Browsing Artículos by Author "Delgado, Sandra"
Now showing items 1-9 of 9
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Galectin-4 N-Terminal Domain: Binding Preferences Toward A and B Antigens with Different Peripheral Core Presentations
Quintana García, Jon Imanol; Delgado, Sandra; Núñez Franco, Reyes; Cañada Vicinay, Francisco Javier; Jiménez Oses, Gonzalo; Jiménez Barbero, Jesús ; Ardá, Ana (Frontiers Media, 2021-04-21)The tandem-repeat Galectin-4 (Gal-4) contains two different domains covalently linked through a short flexible peptide. Both domains have been shown to bind preferentially to A and B histo blood group antigens with different ... -
Molecular Recognition of Glycan-Bearing Glycomacromolecules Presented at Membrane Surfaces by Lectins: An NMR View
Gutiérrez Lete, Marta; Hoffmann, Miriam; Schomann, Nils; Martínez Castillo, Ane; Peccati, Francesca; Konietzny, Patrick B.; Delgado, Sandra; Snyder, Nicole L.; Jiménez Oses, Gonzalo; Abrescia, Nicola G. A.; Ardá, Ana ; Hartmann, Laura; Jiménez Barbero, Jesús (American Chemical Society, 2023-05)Lectin–glycan interactions are at the heart of a multitude of biological events. Glycans are usually presented in a multivalent manner on the cell surface as part of the so-called glycocalyx, where they interact with other ... -
Novel Dextran-Supported Biological Probes Decorated with Disaccharide Entities for Investigating the Carbohydrate-Protein Interactions of Gal-3.
Rahkila, Jani; Ekholm, Filip S.; Ardá, Ana ; Delgado, Sandra; Savolainen, Johannes; Jiménez Barbero, Jesús ; Leino, Reko (Wiley, 2018-11-30)The quest for novel natural-like biomolecular probes that can be used to gain information on biological recognition events is of topical interest to several scientific areas. In particular, the recognition of carbohydrates ... -
Selective C-13-Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins
Moure, María J.; Gimeno, Ana; Delgado, Sandra; Diercks, Tammo; Boons, Geert-Jan; Jiménez Barbero, Jesús ; Ardá, Ana (Wiley, 2021-08-16)A combined chemo-enzymatic synthesis/NMR-based methodology is presented to identify, in unambiguous manner, the distinctive binding epitope within repeating sugar oligomers when binding to protein receptors. The concept ... -
Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins
Lenza, Maria Pia; Oyenarte Santamaría, Iker; Diercks, Tammo; Quintana García, Jon Imanol; Gimeno, Ana; Coelho, Helena; Diniz, Ana; Peccati, Francesca; Delgado, Sandra; Bosch, Alexandre; Valle, Mikel; Millet Aguilar-Galindo, Oscar; Abrescia, Nicola A.G.; Palazón, Asís; Marcelo, Filipa; Jiménez Oses, Gonzalo; Jiménez Barbero, Jesús ; Ardá, Ana ; Ereño Orbea, June (Wiley, 2020-10)The glycan structures of the receptor binding domain of the SARS-CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR. The different possible interacting epitopes have been deeply analysed ... -
Structures of the Inhibitory Receptor Siglec-8 in Complex with a High-Affinity Sialoside Analogue and a Therapeutic Antibody
Lenza, Maria Pia; Atxabal Arrasate, Unai; Nycholat, Corwin; Oyenarte Santamaría, Iker; Franconetti García, Antonio; Quintana García, Jon Imanol; Delgado, Sandra; Núñez Franco, Reyes; Garnica Marroquín, Carmen Teresa; Coelho, Helena; Unione, Luca; Jiménez Oses, Gonzalo; Marcelo, Filipa; Schubert, Mario; Paulson, James C.; Jiménez Barbero, Jesús ; Ereño Orbea, June (American Chemical Society, 2023-01)Human sialic acid binding immunoglobulin-like lectin-8 (Siglec-8) is an inhibitory receptor that triggers eosinophil apoptosis and can inhibit mast cell degranulation when engaged by specific monoclonal antibodies (mAbs) ... -
The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR
Gómez Redondo, Marcos; Delgado, Sandra; Núñez Franco, Reyes; Jiménez Oses, Gonzalo; Ardá, Ana ; Jiménez Barbero, Jesús ; Gimeno, Ana (Royal Society of Chemistry, 2021-06-01)The interaction of human galectin-8 and its two separate N-terminal and C-terminal carbohydrate recognition domains (CRD) to their natural ligands has been analysed using a synergistic combination of experimental NMR and ... -
Unraveling Sugar Binding Modes to DC-SIGN by Employing Fluorinated Carbohydrates
Martínez, José Daniel; Valverde, Pablo; Delgado, Sandra; Romanó, Cecilia; Linclau, Bruno; Reichardt, Niels Christian; Oscarson, Stefan; Ardá, Ana ; Jiménez Barbero, Jesús ; Cañada Vicinay, Francisco Javier (MDPI, 2019-06-25)A fluorine nuclear magnetic resonance (F-19-NMR)-based method is employed to assess the binding preferences and interaction details of a library of synthetic fluorinated monosaccharides towards dendritic cell-specific ... -
Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin-1
Bertuzzi, Sara; Gimeno, Ana; Núñez Franco, Reyes; Bernardo Seisdedos, Ganeko; Delgado, Sandra; Jiménez Oses, Gonzalo; Millet Aguilar-Galindo, Oscar; Jiménez Barbero, Jesús ; Ardá, Ana (Wiley, 2020-12-01)The interaction of human galectin-1 with a variety of oligosaccharides, from di-(N-acetyllactosamine) to tetra-saccharides (blood B type-II antigen) has been scrutinized by using a combined approach of different NMR ...