dc.contributor.author | Miranda Gómez, Jonatan | |
dc.contributor.author | Churruca Ortega, Itziar | |
dc.contributor.author | Fernández Quintela, Alfredo | |
dc.contributor.author | Rodríguez Rivera, Víctor Manuel | |
dc.contributor.author | Macarulla Arenaza, María Teresa | |
dc.contributor.author | Simón Magro, Edurne | |
dc.contributor.author | Portillo Baquedano, María Puy | |
dc.date.accessioned | 2011-05-18T18:20:47Z | |
dc.date.available | 2011-05-18T18:20:47Z | |
dc.date.issued | 2009-12-14 | |
dc.identifier.citation | British Journal of Nutrition 102(11) : 1583-1589 (2009) | es |
dc.identifier.issn | 0007-1145 | |
dc.identifier.uri | http://hdl.handle.net/10810/2545 | |
dc.description.abstract | It has been proposed that young animals and subjects are more responsive to conjugated linoleic acid (CLA) than the adults. Nevertheless, there is very little information concerning the effectiveness of CLA in adult animals. In the present study we aimed to explore the effects of trans-10, cis-12-CLA on body fat accumulation in adult hamsters, as well as on some of the molecular mechanisms described in young animals as responsible for the CLA body fat-lowering effect, such as lipogenesis, lipoprotein lipase (LPL)-mediated fat uptake and thermogenesis. The experiment was conducted with sixteen adult male Syrian Golden hamsters (aged 8 months) fed a high-fat diet supplemented or not with 0·5 % trans-10, cis-12-CLA for 6 weeks. Acetyl-CoA carboxylase (ACX), fatty acid synthase (FAS), LPL, PPARγ, sterol regulatory element-binding protein (SREBP)-1a and SREBP-1c expressions were assessed in subcutaneous and perirenal adipose tissues by real-time RT-PCR. Total and heparin-releasable LPL activities were determined in subcutaneous adipose tissue by fluorimetry and FAS activity by spectrophotometry. Uncoupling protein-1 (UCP1) expression in interscapular brown adipose tissue was assessed by Western blot. Hamsters fed the trans-10, cis-12-CLA diet showed a significant reduction in subcutaneous adipose tissue. No changes were observed in the expression of ACX, FAS, LPL, SREBP-1a, SREBP-1c and PPARγ, nor in total and heparin-releasable LPL and FAS activities. Trans-10, cis-12-CLA induced a significant increase in the amount of UCP1. These results suggest a low responsiveness to trans-10, cis-12-CLA in adults, lower than that in young hamsters. One of the reasons explaining this difference is the lack of effect on LPL. | es |
dc.description.sponsorship | Supported by grants from the Ministerio de Educacion y Ciencia (AGL2005-02 494), Gobierno Vasco (GIC07/120-IT-265-07; CTP 05/R5), University of Pais Vasco (GIU06/82) and CYTED (Programa lberoamericano de Ciencia y Tecnologia para el desarrollo). J. M. is a recipient of a doctoral fellowship from the Ministerio de Educacion y Ciencia. Palm oil was a generous gift from Agra-Unilever Foods Espana S.A. (Leioa, Spain). | es |
dc.language.iso | eng | es |
dc.publisher | Cambridge University Press | es |
dc.relation | info:eu-repo/grantAgreement/MCYT/AGL2005-02 494 | |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | conjugated linoleic acid | es |
dc.subject | adult hamsters | es |
dc.subject | adipose tissue | es |
dc.title | Weak effect of trans-10, cis-12-conjugated linoleic acid on body fat accumulation in adult hamsters | es |
dc.type | info:eu-repo/semantics/article | es |
dc.rights.holder | Copyright © The Authors 2009 | es |
dc.relation.publisherversion | http://journals.cambridge.org/abstract_S0007114509990912 | es |
dc.identifier.doi | 10.1017/S0007114509990912 | |
dc.departamentoes | Farmacia y ciencias de los alimentos | es_ES |
dc.departamentoeu | Farmazia eta elikagaien zientziak | es_ES |
dc.subject.categoria | MEDICINE | |
dc.subject.categoria | NUTRITION AND DIETETICS | |